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bis-TRIS

SKU
B-2433
  • CAS:6976-37-0
  • Formula:C8H19NO5
  • MW:209.2 Da
  • Appearance:White crystalline powder
  • Purity:≥99.0%

2

1

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SDS
CofA
Product Name Qty
bis-TRIS
100 g
$87.15
bis-TRIS
500 g
$398.99
bis-TRIS
1 kg
$609.30
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Description

bis-Tris is a zwitterionic biological buffer used for biological and biochemical research. It is highly soluble in water and exhibits only a very small shift in dissociation constant. Its functional groups are involved to two structural families, the Bis(2-hydroxyethly) amine family and the Tris family. This buffer interacts with human liver fatty acid protein (FAB) and affects protein dynamics. Suitable for fraction of hemoglobin. The useful pH range for the buffer is 5.8-7.2 (pKa of 6.5 at 25℃)

Application

bis-Tris is used as a buffer for anion exchange chromatography; running buffer, gel buffer and sample buffer with different types of electrophoresis. Alternative to cacodylate, maleic acid and citrate. 

Reference

• The uses of bis-Tris buffer 

Specifications

• pH, 1.0 M aqueous solution @ 25ºC: 9.5-11.0
• pKa @ 20ºC: 6.0-7.0
• UV Absorbance @280nm, 1.0 M in H2O: ≤ 0.40

Not for human therapeutic use or for medicinal purposes. For research applications only.

More Information
Alternate Name/Synonyms
Bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane
SKU
B-2433
CAS #
6976-37-0
Chemical Name
2-[bis(2-hydroxyethyl)amino]-2-(hydroxymethyl)propane-1,3-diol
Chemical Formula
C8H19NO5
Molecular Weight
209.2 Da
Appearance
White crystalline powder
Purity
≥99.0%
Solubility
Water: clear colorless solution
Melting Point
102-106°C
Storage Temp
Room temperature
THERAPEUTIC AREA
Not Applicable
USE
BIS-Tris is an inert, zwitterionic buffer that exhibits only a very small shift in dissociation constant with respect to temperature. Suitable for fractionation of hemoglobin. The useful pH range for the buffer is 5.8-7.2. (pKa of 6.5 at 25°C.)
MDL NUMBER
MFCD00002853
CHEMACX
X1006408-1
INCHI
InChI=1S/C8H19NO5/c10-3-1-9(2-4-11)8(5-12,6-13)7-14/h10-14H,1-7H2
SMILES
C(CO)N(CCO)C(CO)(CO)CO
Peptide sequence
Not Applicable
RTECS
Not Applicable
UN #'S
Not Applicable
PACKING GROUP
Not Applicable
HAZARD CLASS
Not Applicable
Handling
Use in a chemical fume hood, with air supplied by an independent system. Avoid inhalation, contact with eyes, skin and clothing. Avoid the formation of dust and aerosols. Use in a well-ventilated area. Keep away from sources of ignition. Avoid prolonged or repeated exposure.
GHS PICTOGRAMS
Handling Use in a chemical fume hood, with air supplied by an independent system. Avoid inhalation, contact with eyes, skin and clothing. Avoid the formation of dust and aerosols. Use in a well-ventilated area. Keep away from sources of ignition. Avoid prolonged or repeated exposure.
UN #'S Not Applicable
Packing Group Not Applicable
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