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  • Blasticidin S HCl Solution in HEPES buffer (10 mg/mL)

Blasticidin S HCl Solution in HEPES buffer (10 mg/mL)

SKU
B-1247-SOL
  • CAS:3513-03-9
  • Formula:C17H26N8O5•HCl
  • MW:458.9 Da
  • Appearance:Clear solution
  • Purity:≥98.0%

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SDS
CofA
Product Name Qty
Blasticidin S.HCl Solution in HEPES buffer 10mg/ml
1x1ml
$37.64
Blasticidin S.HCl Solution in HEPES buffer 10mg/ml
5x1 ml
$156.83
Blasticidin S.HCl Solution in HEPES buffer 10mg/ml
10x1 ml
$227.92
Blasticidin S.HCl Solution in HEPES buffer 10mg/ml
5x2 ml
$230.01
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*AG Scientific's Blasticidin S HCl solution is a sterile filterate solution.*

Description

Blasticidin S HCL is an aminoacyl nucleoside antibiotic isolated from Streptomyces griseochromogenes. It functions by inhibiting peptide bond formation at the ribosome in both eukaryotic and prokaryotic cells. It blocks hydrolysis of peptidyl-tRNA due to the large ribosomal subunit's increased affinity for tRNA. Blasticidin S HCL has two deaminase genes: BSD from Aspergillus terreus and bsr from Bacillus cereus, where resistance is shown converting Blasticidin S HCL into a nontoxic deaminohydroxy derivative.

 Applications

• Used as a selection agent for bls, bsr, or BSD resistance genes
• Prevents cell culture contamination
• Effective in antiviral, antitumor, and antifungal properties
• New role in Genome editing utilizing the CRISPR/Cas9 system.
• Gene selection antibiotic

Concentration: 10 mg/ml in HEPES Buffer

More Information
Alternate Name/Synonyms
Bla-S; Blasticidin S
SKU
B-1247-SOL
CAS #
3513-03-9
Chemical Name
(2S,3S,6R)-3-[[(3S)-3-amino-5-[carbamimidoyl(methyl)amino]pentanoyl]amino]-6-(4-amino-2-oxopyrimidin-1-yl)-3,6-dihydro-2H-pyran-2-carboxylic acid;hydrochloride
Chemical Formula
C17H26N8O5•HCl
Molecular Weight
458.9 Da
Appearance
Clear solution
Purity
≥98.0%
Solubility
Water: clear and faint yellow solution (5 mg/mL)
Storage Temp
+4ºC, desiccated
THERAPEUTIC AREA
Antibacterial
USE
A nucleoside antibiotic supplied at 10 mg/mL in HEPES Buffer for use as a selection antibiotic. Blasticidin S is a nucleoside antibiotic isolated from Streptomyces griseochromogenes which inhibits protein synthesis in both prokaryotic and eukaryotic cells. Blasticidin S acts quickly by inhibiting peptide bond formation by the ribosome. Resistance is conferred by expression of one of two Blasticidin S deaminase genes: BSD from Aspergillus terreus or bsr from Bacillus cereus. These deaminases convert Blasticidin S to a nontoxic deaminohydroxy derivative.
Merck Index
14, 1316
MDL NUMBER
MFCD02091640
CHEMACX
X1339381-2
INCHI
InChI=1S/C17H26N8O5.ClH/c1-24(16(20)21)6-4-9(18)8-12(26)22-10-2-3-13(30-14(10)15(27)28)25-7-5-11(19)23-17(25)29;/h2-3,5,7,9-10,13-14H,4,6,8,18H2,1H3,(H3,20,21)(H,22,26)(H,27,28)(H2,19,23,29);1H
SMILES
[H]/N=C(N)/N(C)CCC(CC(=O)NC1C=CC(OC1C(=O)O)n2ccc(nc2=O)N)N.Cl
Handling
Avoid contact with skin, eyes or clothing. Use personal protective equipment as required. Wash contaminated clothing before reuse. Do not breathe dust/fume/gas/mist/vapors/spray. Do not eat, drink or smoke when using this product.
Citations
Human Apolipoprotein E Is Required for Infectivity and Production of Hepatitis C Virus in Cell Culture, Kyung-Soo Chang, Jieyun Jiang, Zhaohui Cai, and Guangxiang Luo, J. Virol., Dec 2007; 81: 13783 - 13793.
Citations_Hyperlink
http://jvi.asm.org/cgi/reprint/81/24/13783
msds 1
B-1247-SOL, Blasticidin S HCl Solution, SDS, diamond format.pdf
Certificate of Analysis 1
B-1247-SOL, J1105.pdf
Certificate of Analysis 2
B-1247-SOL, H1188.pdf
Certificate of Analysis 3
B-1247-SOL, H1183E.pdf
GHS PICTOGRAMS
Handling Avoid contact with skin, eyes or clothing. Use personal protective equipment as required. Wash contaminated clothing before reuse. Do not breathe dust/fume/gas/mist/vapors/spray. Do not eat, drink or smoke when using this product.
Citations Phosphatidic acid phospholipase A1 mediates ER-Golgi transit of a family of G protein-coupled receptors
Human apolipoprotein e is required for infectivity and production of hepatitis C virus in cell culture
Replication of hepatitis C virus (HCV) RNA in mouse embryonic fibroblasts: protein kinase R (PKR)-dependent and PKR-independent mechanisms for controlling HCV RNA replication and mediating interferon activities
Robust production of infectious hepatitis C virus (HCV) from stably HCV cDNA-transfected human hepatoma cells
Interaction study of the male specific lethal (MSL) complex and trans-acting dosage effects in metafemales of Drosophila melanogaster
Cell-Cell Contact Area Affects Notch Signaling and Notch-Dependent Patterning
Exploring the Interaction between DdCenB and DdRad4 and Their Roles in D. discoideum DNA Repair
Decreased K13 Abundance Reduces Hemoglobin Catabolism and Proteotoxic Stress, Underpinning Artemisinin Resistance
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