Blasticidin S HCl

SKU
B-1247
  • CAS:3513-03-9
  • Formula:C17H26N8O5•HCl
  • MW:458.9 Da
  • Appearance:White powder
  • Purity:≥98.0%

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Starting at $139.02

Product Name Qty
Blasticidin S HCl
25 mg
$139.02
Blasticidin S HCl
1 g
$1,409.38
Blasticidin S HCl
100 mg
$200.90

Description

Blasticidin S HCL is an antibiotic that demonstrates a strong inhibitory effect on protein synthesis in eukaryotic and prokaryotic cells. Blasticidin causes tRNA to bond more strongly to the P site of the large ribosomal subunit while simultaneously deforming the tRNA, thus inhibiting proper translation. The ultimate result of this process is a significant suppression of the hydrolysis of peptidyl-tRNA along with inhibition of peptide bond formation. Isolated from Streptomyces griseochromogenes.

Applications

Aside from its antibiotic effects, blasticidin can be used to select for blasticidin resistance genes, which have undergone considerable study in recent years. One resistance gene, blasticidin S deaminase, has been used as a selection marker for genetic transformation and genome editing. The antibiotic has also been used as a selection factor in studies of forced genetic expression in anticancer therapy, specifically of the CSR1 gene.

• Used as a selection agent for bls, bsr, or BSD resistance genes 
• Prevents cell culture contamination
• Effective in antiviral, antitumor, and antifungal properties
• New role in Genome editing utilizing the CRISPR/Cas9 system. 
• Gene selection antibiotic

 

Blasticidin S HCl Solution (10 mg/mL) available here.

 

More Information
Alternate Name/Synonyms
Blasticidin S; Bla-S
SKU
B-1247
CAS #
3513-03-9
Chemical Name
(2S,3S,6R)-3-[[(3S)-3-amino-5-[carbamimidoyl(methyl)amino]pentanoyl]amino]-6-(4-amino-2-oxopyrimidin-1-yl)-3,6-dihydro-2H-pyran-2-carboxylic acid;hydrochloride
Chemical Formula
C17H26N8O5•HCl
Molecular Weight
458.9 Da
Appearance
White powder
Purity
≥98.0%
Solubility
Soluble in water. Clear and faint yellow solution at 5 mg/ml in water. Insoluble in organic solvents.
Melting Point
218.0-225.0 °C
Storage Temp
+4°C, desiccated
THERAPEUTIC AREA
Antibacterial
USE
Blasticidin is an efficient selection antibiotic that acts on both eukaryotic and prokaryotic cells. Blasticidin is a peptidyl nucleoside antibiotic isolated from Streptomyces griseochromogenes that inhibits protein synthesis by interfering with the peptide-bond formation in the ribosomal machinery. In biological research, specifically genetic engineering, it is used to select transformed cells which have been engineered to carry a resistance gene for blasticidin. In short, DNA of interest is fused to DNA encoding a resistance gene, and then is transformed into cells. After allowing time for recovery and for cells to begin transcribing and translating their new DNA, blasticidin is added. Now only the cells that have the new DNA can grow. Resistance to blasticidin is conferred by the blasticidin resistance gene from Bacillus cereus (bsr), which codes for blasticidin-S deaminase. Resistance to blasticidin is also conferred by the blastcidin S acetyltransferase gene (bls) from Streptoverticillum sp, and the blasticidin S deaminase gene (BSD) from Apergillus terreus. Cell death induced by blasticidin occurs rapidly, allowing for selection of transfected cell lines carrying a blasticidin resistance gene within one week.
MDL NUMBER
MFCD02091640
CHEMACX
X1339381-2
INCHI
InChI=1S/C17H26N8O5.ClH/c1-24(16(20)21)6-4-9(18)8-12(26)22-10-2-3-13(30-14(10)15(27)28)25-7-5-11(19)23-17(25)29;/h2-3,5,7,9-10,13-14H,4,6,8,18H2,1H3,(H3,20,21)(H,22,26)(H,27,28)(H2,19,23,29);1H
SMILES
[H]/N=C(N)/N(C)CCC(CC(=O)NC1C=CC(OC1C(=O)O)n2ccc(nc2=O)N)N.Cl
Peptide sequence
Not Applicable
RTECS
Not Applicable
UN #'S
UN 2811
PACKING GROUP
II
HAZARD CLASS
Not Applicable
Handling
Avoid contact with skin, eyes or clothing. Use personal protective equipment as required. Wash contaminated clothing before reuse. Do not breathe dust/fume/gas/mist/vapors/spray. Do not eat, drink or smoke when using this product. Corrosive hazard. Wear protective gloves/clothing and eye/face protection. Thermal decomposition can lead to release of toxic/corrosive gases and vapors.
Special Shipping
Yes
Citations
VIRUS-CELL INTERACTIONS: Human Apolipoprotein E Is Required for Infectivity and Production of Hepatitis C Virus in Cell Culture Kyung-Soo Chang, Jieyun Jiang, Zhaohui Cai, and Guangxiang Luo J. Virol., Dec 2007; 81: 13783 - 13793.
Citations_Hyperlink
http://jvi.asm.org/cgi/reprint/81/24/13783
Certificate of Analysis 1
Certificate of Analysis 2
Certificate of Analysis 3
GHS PICTOGRAMS
Handling Avoid contact with skin, eyes or clothing. Use personal protective equipment as required. Wash contaminated clothing before reuse. Do not breathe dust/fume/gas/mist/vapors/spray. Do not eat, drink or smoke when using this product. Corrosive hazard. Wear protective gloves/clothing and eye/face protection. Thermal decomposition can lead to release of toxic/corrosive gases and vapors.
UN #'S UN 2811
Packing Group II
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