Blasticidin S is an antibiotic used by scientists in bio-research to select cells modified by genetic engineering. It inhibits the growth of a wide range of prokaryotic and eukaryotic by interfering with protein synthesis cells. Blasticidin S allows the selection and maintenance of cells expressing the blasticidin-resistance gene. Blasticidin S is a peptidyl nucleoside antibiotic isolated from the culture broth of Streptomyces griseochromogenes.
Blasticidin S specifically inhibits protein synthesis in both prokaryotes and eukaryotes through inhibition of peptide-bond formation in the ribosomal machinery. It is used to select transfected cells carrying bsr or BSD resistance genes. Blasticidin S specifically inhibits protein synthesis in both prokaryotes and eukaryotes through inhibition of peptide-bond formation in the ribosomal machinery.
How do you determine Blasticidin sensitivity?
- Plate cells at approximately 25% confluency. Prepare a set of 6 plates. Allow cells to adhere overnight.
- The next day, substitute culture medium with medium containing varying concentrations of Blasticidin ( e.g. 0,2,4,6,8,10 µg / mL Blasticidin)
- Replenish the selective media every 3-4 days, and observe the percentage of surviving cells.
- Determine the appropriate concentration of Blasticidin that kills the cells within 10-14 days after addition of the antibiotic.
What mammalian cell lines work with Blasticidin S?
| Cell Type | Selection Concentration ( µg/ml) |
| 10T1/2 cells | ~10 µg/ml |
| 293 HEK | 3-10 µg/ml |
| 293T Lα | ~10 ug/ml |
| A2780 | ~5 µg/ml |
| A549 | 2.5 to 10 ug/mL |
| ASK | 2.5-10 µg/ml |
| B16 | 3-10 µg/ml |
| CHO | 5-10 µg/ml |
| COS-1 | 3-10 µg/ml |
| COLO 320DM | ~10 µg/ml |
| CSII-EF-RfA | ~ 5 µg/ml |
| CV1-P | 2.5-6 µg/ml |
| D. discoideum coupled w/ Klebsiella aerogenes | ~ 10 µg/ml |
| DH1 | ~10 µg/ml |
| DF-1 | 10 ug/ml |
| EBV | ~5 µg/ml |
| Giardia cells | ~75 ug/ml |
| HAV | 1-3 µg/ml |
| HEK293T | 2.5 to 10 ug/mL |
| HeLa-S3 | 2.5 to 10 ug/mL |
| HeLa | 2.5 to 10 ug/mL |
| HIV | 10 µg/ml |
| HMEC-1 | 2.5-6 µg/ml |
| HMX44A | ~10 µg/ml |
| HT1080 | 2.5 to 10 ug/mL |
| FIV | 10 µg/ml |
| iPS | ~ 10 ug/ml |
| JH10 | ~10 µg/ml |
| IMCD | ~5 ug/ml |
| L929 | 2.5 to 10 ug/mL |
| PC1.0 | 10-30 µg/ml |
| MHOM/IL/80/Friedlin | 10 µg/ml |
| N2a | 2.5 to 10 ug/mL |
| NIH-3T3 | 2.5 to 10 ug/mL |
| Nannochloropsis sp (algae) | ~5 ug/mL |
| Plasmodium falciparum | 2.5-5.0 ug/ml |
| SHK-1 | 2.5-10 µg/ml |
| S2 Drosophila | ~ 5 µg/ml |
| S. cerevisiae | ~25 µg/ml |
| TRE5-A | ~5 µg/ml |
| T3-L1 | 2.5-10 µg/ml |
| thraustochytrids | 100 ug/ml |
| TO | 2.5-10 µg/ml |
| U2OS | 2.5 to 10 ug/mL |