5-Bromo-3-Indolyl-β-D-Galactopyranoside
97753-82-7
374.2
C14H16BrNO6
DMSO, DMF
-20°C
Bluo-Gal (5-Bromo-3-indolyl β-D-galactopyranoside) is a chromogenic substrate for β-galactosidase (LacZ), used to determine the presence or absence of a cloned DNA inserted in bacteria growing on agar plates.
When it is hydrolyzed by β-galactosidase, the resulting product will form a blue precipitate. The precipitate is insoluble in alcohol and xylenes, thus it is suitable for immunoblotting and immunocytochemical assays.
Upon microscopic examination under polarized light, the precipitated crystals emit a strong signal consisting of yellow reflected light. Because of this property, Bluo-Gal has more potent sensitivity of the staining method for β-galactosidase and has optimal morphological resolution.
Bluo-Gal can be used as an alternative of X-Gal. Lac+ colonies grown in the presence of Bluo-Gal turn a deeper blue color more intense than X-Gal, allowing for easy differentiation between lac+ and lac- colonies.
Applications:
- Confirmation of cloned DNA insert in bacteria
- Lac gene detection systems
- Detection of β-gal activity by electron microscopy
- Immunoblotting
- Immunocytochemical assays
- Used in conjunction with IPTG for blue-white screening
0.1 lbs
Research or further manufacturing use only, not for food or drug use.
