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Bluo-Gal, 100 MG

Price$ 53.25
  • SKU: X-1016-100MG
  • Pack Size: 100 MG
Quantity
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Total price: $ 772.00

This item: Bluo-Gal, 100 MG $ 53.25

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5-Bromo-3-Indolyl-β-D-Galactopyranoside

97753-82-7

374.2

C14H16BrNO6

DMSO, DMF

-20°C


Bluo-Gal (5-Bromo-3-indolyl β-D-galactopyranoside) is a chromogenic substrate for β-galactosidase (LacZ), used to determine the presence or absence of a cloned DNA inserted in bacteria growing on agar plates.
 

When it is hydrolyzed by β-galactosidase, the resulting product will form a blue precipitate. The precipitate is insoluble in alcohol and xylenes, thus it is suitable for immunoblotting and immunocytochemical assays.
 

Upon microscopic examination under polarized light, the precipitated crystals emit a strong signal consisting of yellow reflected light. Because of this property, Bluo-Gal has more potent sensitivity of the staining method for β-galactosidase and has optimal morphological resolution.
 

Bluo-Gal can be used as an alternative of X-Gal. Lac+ colonies grown in the presence of Bluo-Gal turn a deeper blue color more intense than X-Gal, allowing for easy differentiation between lac+ and lac- colonies.


Applications:

  • Confirmation of cloned DNA insert in bacteria
  • Lac gene detection systems
  • Detection of β-gal activity by electron microscopy
  • Immunoblotting
  • Immunocytochemical assays
  • Used in conjunction with IPTG for blue-white screening
 

 

0.1 lbs



Research or further manufacturing use only, not for food or drug use.

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