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Bluo-GAL

SKU
X-1016
  • CAS:97753-82-7
  • Formula:C14H16BrNO6
  • MW:374.2 Da
  • Appearance:White Powder
  • Purity:≥99% (TLC)

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SDS
CofA
Product Name Qty
Bluo-GAL
100 mg
$44.88
Bluo-GAL
1 g
$147.90
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Bluo-Gal is a chromogenic substrate for β-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. When Bluo-Gal is hydrolyzed by β-galactosidase, the resulting product will form a blue precipitate. This precipitate is insoluble in alcohol and xylenes, thus making it suitable for immunoblotting and immunocytochemical assays. Lac+ colones grown in the presence of Bluo-Gal turn a deeper blue color more intense than X-Gal, allowing for easy differentiation between lac+ and lac- colonies.

 

 

More Information
Alternate Name/Synonyms
Blue-gal; 5-Bromo-3-indolyl β-D-galactopyranoside
SKU
X-1016
CAS #
97753-82-7
Chemical Name
(2S,3R,4S,5R,6R)-2-[(5-bromo-1H-indol-3-yl)oxy]-6-(hydroxymethyl)oxane-3,4,5-triol
Chemical Formula
C14H16BrNO6
Molecular Weight
374.2 Da
Appearance
White Powder
Purity
≥99% (TLC)
Solubility
Clear and colorless solution (Sol. 2%, DMF)
Storage Temp
-20°C, desiccated
THERAPEUTIC AREA
Not Applicable
USE
Bluo-Gal is a substrate for beta-galactosidase that is converted, by the enzyme, into an insoluble indigoblue chromophore. It is also used in Lac gene detection systems, in immunoblotting and immunocytochemical assays.
MDL NUMBER
MFCD00063691
CHEMACX
X1076984-7
INCHI
InChI=1S/C14H16BrNO6/c15-6-1-2-8-7(3-6)9(4-16-8)21-14-13(20)12(19)11(18)10(5-17)22-14/h1-4,10-14,16-20H,5H2/t10-,11+,12+,13-,14-/m1/s1
SMILES
c1cc2c(cc1Br)c(c[nH]2)O[C@H]3[C@@H]([C@H]([C@H]([C@H](O3)CO)O)O)O
Handling
Use only in area provided with appropriate exhaust ventilation. Keep away from heat and source of ignition. Empty containers pose a fire risk, evaporate residue under fume hood. Ground all equipment containing material. Do not breathe dust.
msds 1
X-1016, Bluo-Gal, SDS, diamond format.pdf
Certificate of Analysis 1
X-1016, J1086A.pdf
Certificate of Analysis 2
X-1016, J1086.pdf
Certificate of Analysis 3
X-1016, J1071.pdf
GHS PICTOGRAMS
Handling Use only in area provided with appropriate exhaust ventilation. Keep away from heat and source of ignition. Empty containers pose a fire risk, evaporate residue under fume hood. Ground all equipment containing material. Do not breathe dust.
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