Bluo-GAL

SKU

X-1016

CAS:97753-82-7
Formula:C₁₄H₁₆BrNO₆
MW:374.2 Da
Appearance:White Powder
Purity:≥99% (TLC)

SDS

CofA

Product Name	Qty
Bluo-GAL 100 mg $44.00
Bluo-GAL 1 g $145.00

Details

Bluo-Gal is a chromogenic substrate for β-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. When Bluo-Gal is hydrolyzed by β-galactosidase, the resulting product will form a blue precipitate. This precipitate is insoluble in alcohol and xylenes, thus making it suitable for immunoblotting and immunocytochemical assays. Lac+ colones grown in the presence of Bluo-Gal turn a deeper blue color more intense than X-Gal, allowing for easy differentiation between lac+ and lac- colonies.

Specifications

More Information

Alternate Name/Synonyms

Blue-gal; 5-Bromo-3-indolyl β-D-galactopyranoside

SKU

X-1016

CAS #

97753-82-7

Chemical Name

(2S,3R,4S,5R,6R)-2-[(5-bromo-1H-indol-3-yl)oxy]-6-(hydroxymethyl)oxane-3,4,5-triol

Chemical Formula

C₁₄H₁₆BrNO₆

Molecular Weight

374.2 Da

Appearance

White Powder

Purity

≥99% (TLC)

Solubility

Clear and colorless solution (Sol. 2%, DMF)

Storage Temp

-20°C, desiccated

THERAPEUTIC AREA

Not Applicable

USE

Bluo-Gal is a substrate for beta-galactosidase that is converted, by the enzyme, into an insoluble indigoblue chromophore. It is also used in Lac gene detection systems, in immunoblotting and immunocytochemical assays.

MDL NUMBER

MFCD00063691

CHEMACX

X1076984-7

INCHI

InChI=1S/C14H16BrNO6/c15-6-1-2-8-7(3-6)9(4-16-8)21-14-13(20)12(19)11(18)10(5-17)22-14/h1-4,10-14,16-20H,5H2/t10-,11+,12+,13-,14-/m1/s1

SMILES

c1cc2c(cc1Br)c(c[nH]2)O[C@H]3[C@@H]([C@H]([C@H]([C@H](O3)CO)O)O)O

Handling

Use only in area provided with appropriate exhaust ventilation. Keep away from heat and source of ignition. Empty containers pose a fire risk, evaporate residue under fume hood. Ground all equipment containing material. Do not breathe dust.

msds 1

X-1016, Bluo-Gal, SDS, diamond format.pdf

Certificate of Analysis 1

X-1016, J1086A.pdf

Certificate of Analysis 2

X-1016, J1086.pdf

Certificate of Analysis 3

X-1016, J1071.pdf

Reviews

Safety

GHS PICTOGRAMS
Handling	Use only in area provided with appropriate exhaust ventilation. Keep away from heat and source of ignition. Empty containers pose a fire risk, evaporate residue under fume hood. Ground all equipment containing material. Do not breathe dust.

Citations

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