CHAPS Zwitterionic, High Purity

SKU

C-1019

CAS:75621-03-3
Formula:C₃₂H₅₈N₂O₇S
MW:614.9 Da
Appearance:White solid
Purity:≥98%

SDS

CofA

Starting at $44.08

Product Name	Qty
CHAPS, High Purity 10 g $55.35
CHAPS, High Purity 5 g $44.08
CHAPS, High Purity 50 g $206.68
CHAPS, High Purity 100 g $281.88
CHAPS, High Purity 1 kg $2,204.64

Details

Description

CHAPS is a zwitterionic detergent that is widely used in the protein purification process. As part of this class of detergents, CHAPS contains both a positive and a negative group, but does not have a net overall charge. CHAPS has a relatively low biomembrane affinity, but is effective in inducing hemolysis and protein solubilization within specific concentration ranges. CHAPS is classified as a non-denaturing surfactant with a critical micelle concentration of 6-10mM.

Applications

In the laboratory, CHAPS and other zwitterionic detergents can be used for inducing cell lysis prior to protein isolation. It can also be used as a buffer in the 2D gel electrophoresis process, where it is in some cases favored over anionic detergents such as SDS. CHAPS can be removed from solutions through dialysis prior to protein analysis. Another technique leveraging a specialized resin and a spin column can also be used to efficiently remove CHAPS

• For monitoring chemical reactions or protein-protein binding using UV/VIS spectroscopy
• For ion exchange chromatography and isoelectric
• For membrane permeabilization of cells
• As a component in denaturing buffer in enzyme characterization

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CHAPS Solution available here

Specifications

More Information

Alternate Name/Synonyms

CHAPS

SKU

C-1019

CAS #

75621-03-3

Chemical Name

3-[dimethyl-[3-[[(4R)-4-[(3R,5S,7R,8R,9S,10S,12S,13R,14S,17R)-3,7,12-trihydroxy-10,13-dimethyl-2,3,4,5,6,7,8,9,11,12,14,15,16,17-tetradecahydro-1H-cyclopenta[a]phenanthren-17-yl]pentanoyl]amino]propyl]azaniumyl]propane-1-sulfonate

Chemical Formula

C₃₂H₅₈N₂O₇S

Molecular Weight

614.9 Da

Appearance

White solid

Purity

≥98%

Solubility

Soluble in DMSO or DMF

Melting Point

157 °C

Preparation

N/A

Storage Temp

Room temperature

THERAPEUTIC AREA

Not Available

USE

CHAPS is often used as a detergent in the solubilization and purification of membrane proteins for several advantageous reasons. CHAPS detergent is non-denaturing to membrane proteins, can solubilize proteins, disaggregate protein-protein interactions and is electrically neutral. CHAPS is also useful in ion exchange chromatography and isoelectric focusing as it is zwitterionic and does not exhibit a net charge between pH 2 to 12. The critical micelle concentration of CHAPS is 6-10mM.

Merck Index

13.2052.2001

MDL NUMBER

MFCD00012116

CHEMACX

X1016609-1

INCHI

InChI=1S/C32H58N2O7S/c1-21(8-11-29(38)33-14-6-15-34(4,5)16-7-17-42(39,40)41)24-9-10-25-30-26(20-28(37)32(24,25)3)31(2)13-12-23(35)18-22(31)19-27(30)36/h21-28,30,35-37H,6-20H2,1-5H3,(H-,33,38,39,40,41)/t21-,22+,23-,24-,25+,26+,27-,28+,30+,31+,32-/m1/s1

SMILES

C[C@H](CCC(=O)NCCC[N+](C)(C)CCCS(=O)(=O)[O-])[C@H]1CC[C@@H]2[C@@]1([C@H](C[C@H]3[C@H]2[C@@H](C[C@H]4[C@@]3(CC[C@H](C4)O)C)O)O)C

Peptide sequence

Not Applicable

RTECS

Not Applicable

UN #'S

Not Applicable

PACKING GROUP

Not Applicable

HAZARD CLASS

Not Applicable

Handling

Ensure adequate ventilation, especially in confined areas. Use personal protective equipment as required. Use with local exhaust ventilation. Do not breathe dust/fume/gas/mist/vapors/spray. Handle in accordance with good industrial hygiene and safety practice. Corrosive hazard. Wear protective gloves/clothing and eye/face protection.

msds 1

C-1019, CHAPS, SDS, diamond format.pdf

Certificate of Analysis 1

C-1019, J1303.pdf

Certificate of Analysis 2

C-1019, J1219.pdf

Certificate of Analysis 3

C-1019, J1051A.pdf

Reviews

Safety

GHS PICTOGRAMS
Handling	Ensure adequate ventilation, especially in confined areas. Use personal protective equipment as required. Use with local exhaust ventilation. Do not breathe dust/fume/gas/mist/vapors/spray. Handle in accordance with good industrial hygiene and safety practice. Corrosive hazard. Wear protective gloves/clothing and eye/face protection.
UN #'S	Not Applicable
Packing Group	Not Applicable

Citations

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