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  • CHAPS Zwitterionic, High Purity

CHAPS Zwitterionic, High Purity

SKU
C-1019
  • CAS:75621-03-3
  • Formula:C32H58N2O7S
  • MW:614.9 Da
  • Appearance:White solid
  • Purity:≥98%
Product Name Qty
CHAPS, High Purity
5 g
$54.26
CHAPS, High Purity
10 g
$77.22
CHAPS, High Purity
50 g
$234.78
CHAPS, High Purity
100 g
$339.12
CHAPS, High Purity
1 kg
$2,504.30
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Description

CHAPS is a zwitterionic detergent that is widely used in the protein purification process. As part of this class of detergents, CHAPS contains both a positive and a negative group, but does not have a net overall charge. CHAPS has a relatively low biomembrane affinity, but is effective in inducing hemolysis and protein solubilization within specific concentration ranges. CHAPS is classified as a non-denaturing surfactant with a critical micelle concentration of 6-10mM.

Applications

In the laboratory, CHAPS and other zwitterionic detergents can be used for inducing cell lysis prior to protein isolation. It can also be used as a buffer in the 2D gel electrophoresis process, where it is in some cases favored over anionic detergents such as SDS. CHAPS can be removed from solutions through dialysis prior to protein analysis. Another technique leveraging a specialized resin and a spin column can also be used to efficiently remove CHAPS

• For monitoring chemical reactions or protein-protein binding using UV/VIS spectroscopy
• For ion exchange chromatography and isoelectric
• For membrane permeabilization of cells
• As a component in denaturing buffer in enzyme characterization

 

*Inquire for bulk pricing (kg).

CHAPS Solution available here

 

More Information
Alternate Name/Synonyms
CHAPS
SKU
C-1019
CAS #
75621-03-3
Chemical Name
3-[dimethyl-[3-[[(4R)-4-[(3R,5S,7R,8R,9S,10S,12S,13R,14S,17R)-3,7,12-trihydroxy-10,13-dimethyl-2,3,4,5,6,7,8,9,11,12,14,15,16,17-tetradecahydro-1H-cyclopenta[a]phenanthren-17-yl]pentanoyl]amino]propyl]azaniumyl]propane-1-sulfonate
Chemical Formula
C32H58N2O7S
Molecular Weight
614.9 Da
Appearance
White solid
Purity
≥98%
Solubility
Soluble in DMSO or DMF
Melting Point
157 °C
Storage Temp
Room temperature
THERAPEUTIC AREA
Not Available
USE
CHAPS is often used as a detergent in the solubilization and purification of membrane proteins for several advantageous reasons. CHAPS detergent is non-denaturing to membrane proteins, can solubilize proteins, disaggregate protein-protein interactions and is electrically neutral. CHAPS is also useful in ion exchange chromatography and isoelectric focusing as it is zwitterionic and does not exhibit a net charge between pH 2 to 12. The critical micelle concentration of CHAPS is 6-10mM.
Merck Index
13.2052.2001
MDL NUMBER
MFCD00012116
CHEMACX
X1016609-1
INCHI
InChI=1S/C32H58N2O7S/c1-21(8-11-29(38)33-14-6-15-34(4,5)16-7-17-42(39,40)41)24-9-10-25-30-26(20-28(37)32(24,25)3)31(2)13-12-23(35)18-22(31)19-27(30)36/h21-28,30,35-37H,6-20H2,1-5H3,(H-,33,38,39,40,41)/t21-,22+,23-,24-,25+,26+,27-,28+,30+,31+,32-/m1/s1
SMILES
C[C@H](CCC(=O)NCCC[N+](C)(C)CCCS(=O)(=O)[O-])[C@H]1CC[C@@H]2[C@@]1([C@H](C[C@H]3[C@H]2[C@@H](C[C@H]4[C@@]3(CC[C@H](C4)O)C)O)O)C
Peptide sequence
Not Applicable
RTECS
Not Applicable
UN #'S
Not Applicable
PACKING GROUP
Not Applicable
HAZARD CLASS
Not Applicable
Handling
Ensure adequate ventilation, especially in confined areas. Use personal protective equipment as required. Use with local exhaust ventilation. Do not breathe dust/fume/gas/mist/vapors/spray. Handle in accordance with good industrial hygiene and safety practice. Corrosive hazard. Wear protective gloves/clothing and eye/face protection.
msds 1
C-1019, CHAPS, SDS, diamond format, 2020.pdf
Certificate of Analysis 1
C-1019, L1463.pdf
Certificate of Analysis 2
C-1019, L1318.pdf
Certificate of Analysis 3
C-1019, L1002A.pdf
GHS PICTOGRAMS
HandlingEnsure adequate ventilation, especially in confined areas. Use personal protective equipment as required. Use with local exhaust ventilation. Do not breathe dust/fume/gas/mist/vapors/spray. Handle in accordance with good industrial hygiene and safety practice. Corrosive hazard. Wear protective gloves/clothing and eye/face protection.
UN #'SNot Applicable
Packing GroupNot Applicable
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