- MW:154.25 Da
- Appearance:White crystalline powder
Dithiothreitol (DTT), is a small-molecule redox reagent, known as Cleland's reagent, that forms a six-member ring with an internal disulfide bond. It has a redox potential of -0.33 V at pH 7. The reduction of typical disulfide bond proceeds by two sequential thiol-disulfide exchange reactions.
DTT is effective in buffers to reduce protein disulfide bonds prior to SDS-PAGE. It can protect protein sulfhydryls and restore enzyme activity lost by oxidation.
• For reducing or "deprotecting" agent for thiolated DNA
• For reducing disulfide bonds of proteins
• Used as an oxidizing agent
• Used as a component of medium for the demembranation and reactivation of spermatozoa
• Used to prevent intramolecular and intermolecular disulfide bonds from forming between cysteine residues of proteins
Additional Reading from AG Scientific
Not for human therapeutic use or for medicinal purposes. For research applications only.
|Handling||Hygroscopic. Protect from moisture. Protect from light. The pharmacological and toxicological properties of this product have not been fully investigated. Use caution when handling. Do not use this compound if you are not fully trained or are unaware of the hazards involved.|
|UN #'S||UN 3335|