G-418 Sulfate

SKU
G-1033
  • CAS:108321-42-2
  • Formula:C20H40O10N4 • 2H2SO4
  • MW:692.7 Da
  • Appearance:White to off-white powder
  • Purity:≥90% (HPLC)

2

1

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Product Name Qty
G-418.Sulfate
5 g
$99.99
G-418.Sulfate
25 g
$299.99
G-418.Sulfate
1 g
$34.99
G-418.Sulfate
5x1 g
$124.99

Description

G-418 Sulfate Salt (Geneticin) is an aminoglycoside antibiotic that is synthesized from Micromonospora rhodorangea and functions by causing a chain of nonsense mutations during translation. Due to translation G-418 inhibited polypeptide synthesis and protein elongation. G-418 binds to the 80S subunit of the ribosome by blocking the elongation phase of protein synthesis, both in prokaryotic and eukaryotic cells. It also shows activity against protozoa, cecal amoebiasis, and helminthes.

Applications:

• Used routinely in molecular genetics experiments for selecting eukaryotic transformants
• Used in plant and mammalian cell culture as a selectable marker
• Used as a gene selection antibiotic that has a powerful new role in genome editing, utilizing the CRISPR/CAS9 system.
• Ability to inhibit poliovirus and rhinovirus 2A proteinases in vitro

 

More Information
Alternate Name/Synonyms
Geneticin; Glycoside-418 Sulfate
SKU
G-1033
CAS #
108321-42-2
Chemical Name
(2S,3S,4S,5S)-2-[(1S,2S,3R,4S,6R)-4,6-diamino-3-[(2S,3R,4R,5S,6R)-3-amino-4,5-dihydroxy-6-(1-hydroxyethyl)oxan-2-yl]oxy-2-hydroxycyclohexyl]oxy-5-methyl-4-(methylamino)oxane-3,5-diol;sulfuric acid
Chemical Formula
C20H40O10N4 • 2H2SO4
Molecular Weight
692.7 Da
Appearance
White to off-white powder
Purity
≥90% (HPLC)
Solubility
Soluble in water: 100mg/ml, colorless solution
Melting Point
N/A
Preparation
N/A
Storage Temp
Store at +4°C
THERAPEUTIC AREA
Bacterial infections
USE
Geneticin (G418) Sulfate is an aminoglycoside antibiotic that has originally been derived from Micromonospora rhodoranga NRRL 5326. This compound has been shown to cause a bypass of nonsense mutations during translation. Experiments have shown that geneticin sulfate inhibits the incorporation of amino acids into protein and can destroy fibroblasts. Mechanistic studies suggest that this agent affects the 80S ribosome and inhibits the elongation cycle. Further studies report that geneticin sulfate is highly active against amoebae, protozoa, tapeworm, and pinworm exposed murine cells.
Merck Index
N/A
RTECS
CB9378500
Handling
Keep container tightly closed and sealed until ready for use. Containers that have been opened must be carefully resealed and kept upright to prevent leakage. Do not store in unlabeled containers. Use appropriate containment to avoid environmental contamination. Protect from light and moisture! Warning: Harmful! Irritant!
Certificate of Analysis 1
Certificate of Analysis 2
Certificate of Analysis 3
GHS PICTOGRAMS
Handling Keep container tightly closed and sealed until ready for use. Containers that have been opened must be carefully resealed and kept upright to prevent leakage. Do not store in unlabeled containers. Use appropriate containment to avoid environmental contamination. Protect from light and moisture! Warning: Harmful! Irritant!
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