G-418 Sulfate

  • CAS:108321-42-2
  • Formula:C20H40O10N4 • 2H2SO4
  • MW:692.7 Da
  • Appearance:White to off-white powder
  • Purity:≥90% (HPLC)
Product Name Qty
1 g
5 g
5x1 g
25 g


G-418 Sulfate Salt (Geneticin) is an aminoglycoside antibiotic that is synthesized from Micromonospora rhodorangea and functions by causing a chain of nonsense mutations during translation. Due to translation G-418 inhibited polypeptide synthesis and protein elongation. G-418 binds to the 80S subunit of the ribosome by blocking the elongation phase of protein synthesis, both in prokaryotic and eukaryotic cells. It also shows activity against protozoa, cecal amoebiasis, and helminthes.



• Used routinely in molecular genetics experiments for selecting eukaryotic transformants
• Used in plant and mammalian cell culture as a selectable marker
• Used as a gene selection antibiotic that has a powerful new role in genome editing, utilizing the CRISPR/CAS9 system.
• Ability to inhibit poliovirus and rhinovirus 2A proteinases in vitro


Not for human therapeutic use or for medicinal purposes. For research applications only.

More Information
Alternate Name/Synonyms
Geneticin; Glycoside-418 Sulfate
Chemical Name
(2S,3S,4S,5S)-2-[(1S,2S,3R,4S,6R)-4,6-diamino-3-[(2S,3R,4R,5S,6R)-3-amino-4,5-dihydroxy-6-(1-hydroxyethyl)oxan-2-yl]oxy-2-hydroxycyclohexyl]oxy-5-methyl-4-(methylamino)oxane-3,5-diol;sulfuric acid
Chemical Formula
C20H40O10N4 • 2H2SO4
Molecular Weight
692.7 Da
White to off-white powder
≥90% (HPLC)
Soluble in water: 100mg/ml, colorless solution
Storage Temp
Store at +4°C
Bacterial infections
Geneticin (G418) Sulfate is an aminoglycoside antibiotic that has originally been derived from Micromonospora rhodoranga NRRL 5326. This compound has been shown to cause a bypass of nonsense mutations during translation. Experiments have shown that geneticin sulfate inhibits the incorporation of amino acids into protein and can destroy fibroblasts. Mechanistic studies suggest that this agent affects the 80S ribosome and inhibits the elongation cycle. Further studies report that geneticin sulfate is highly active against amoebae, protozoa, tapeworm, and pinworm exposed murine cells.
Keep container tightly closed and sealed until ready for use. Containers that have been opened must be carefully resealed and kept upright to prevent leakage. Do not store in unlabeled containers. Use appropriate containment to avoid environmental contamination. Protect from light and moisture! Warning: Harmful! Irritant!
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HandlingKeep container tightly closed and sealed until ready for use. Containers that have been opened must be carefully resealed and kept upright to prevent leakage. Do not store in unlabeled containers. Use appropriate containment to avoid environmental contamination. Protect from light and moisture! Warning: Harmful! Irritant!
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