HEPES, Free Acid

SKU

H-2431

CAS:7365-45-9
Formula:C₈H₁₈N₂O₄S
MW:238.3 Da
Appearance:White crystalline powder
Purity:≥99.5% (titration, dried basis)

SDS

CofA

Product Name	Qty
HEPES, free acid 100 g $81.59
HEPES, free acid 500 g $239.69
HEPES, free acid 1 kg $356.99
HEPES, free acid 5 kg $1,071.00

Details

Description

HEPES is a zwitterionic N-substituted aminosulfornic acid buffer used in cell culture and as an ampholytic separator to create a pH gradient in isoelectric focusing. It is used as a non-coordinating buffer in solutions with metal ions because it is not likely to form a complex with metal ions.

The buffer is able to form free radicals so it is not suitable for redox reactions. It is reported that oxidative DNA damage can be induced by free radicals generated from HEPES buffer in the presence of Au(III).

In addition, it is demonstrated that HEPES in RPMI medium potently increases hydrogen peroxide and cytotoxic products during exposure to medium to visible light.

Applications

• Buffer

• Cell culture

• BCA assay

Specifications

• Endotoxin (0.2% Solution): ≤ 0.25 EU/mL
• Heavy Metals (as Pb): ≤ 0.001 %
• Iron (Fe): ≤ 0.0005 %
• Loss on Drying: ≤ 1.0 %
• pH (5% in water): 5.0 - 6.5
• pKa @ 20°C: 7.35 - 7.70
• Residue on Ignition: ≤ 0.1 %
• Sulfate: ≤ 0.05 %
• UV Absorbance (1M@260nm): ≤ 0.05
• UV Absorbance (1M@280nm): ≤ 0.05

References

• “Oxidative DNA damage induced by HEPES (2-[4-(2-hydroxyethyl)-1-piperazinyl]ethanesulfonic acid) buffer in the presence of Au(III)”

• “Analysis of the cytotoxic effects of light-exposed hepes-containing culture medium”

Not for human therapeutic use or for medicinal purposes. For research applications only.

Specifications

More Information

Alternate Name/Synonyms

HEPES; Monosodium Salt HEPES

SKU

H-2431

CAS #

7365-45-9

Chemical Name

2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid

Chemical Formula

C₈H₁₈N₂O₄S

Molecular Weight

238.3 Da

Appearance

White crystalline powder

Purity

≥99.5% (titration, dried basis)

Solubility

Water: clear and colorless solution

Melting Point

234 °C

Storage Temp

Room temperature

THERAPEUTIC AREA

Not Applicable

USE

HEPES (4-(2-Hydroxyethyl)piperazine-1-ethane-sulfonic acid) has been described as one of the best all-purpose buffers available for use in biological research. The molecule is zwitterionic at most biological pHs and is most effective as a buffer at pH 6.0 to 8.5. May be used in isoelectric focusing applications and as a buffer in the quantitative and selective measurement of antigen-antibody reactions. HEPES is reportedly superior to NaHCO3 in controlling pH in tissue and organ culture. Not recommended for use with the Folin-Ciocalteu protein assay.

MDL NUMBER

MFCD00006158

CHEMACX

X1001076-9

INCHI

JKMHFZQWWAIEOD-UHFFFAOYSA-N

SMILES

C1CN(CCN1CCO)CCS(=O)(=O)O

Peptide sequence

Not Applicable

RTECS

TL6809000

UN #'S

Not Applicable

PACKING GROUP

Not Applicable

HAZARD CLASS

Not Applicable

Handling

Use only in area provided with appropriate exhaust ventilation. Keep away from heat and source of ignition. Empty containers pose a fire risk, evaporate residue under fume hood. Ground all equipment containing material. Do not breathe dust.

msds 1

H-2431, HEPES, free acid, SDS, diamond format, r01, 2020.pdf

Certificate of Analysis 1

H-2431, K1550.pdf

Certificate of Analysis 2

H-2431, J1048.pdf

Certificate of Analysis 3

H-2431, F1234.pdf

Reviews

Safety

GHS PICTOGRAMS
Handling	Use only in area provided with appropriate exhaust ventilation. Keep away from heat and source of ignition. Empty containers pose a fire risk, evaporate residue under fume hood. Ground all equipment containing material. Do not breathe dust.
UN #'S	Not Applicable
Packing Group	Not Applicable