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HEPES, Free Acid

SKU
H-2431
  • CAS:7365-45-9
  • Formula:C8H18N2O4S
  • MW:238.3 Da
  • Appearance:White crystalline powder
  • Purity:≥99.5% (titration, dried basis)

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SDS
CofA
Product Name Qty
HEPES, free acid
100 g
$81.59
HEPES, free acid
500 g
$239.69
HEPES, free acid
1 kg
$356.99
HEPES, free acid
5 kg
$1,071.00
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Description

HEPES is a zwitterionic N-substituted aminosulfornic acid buffer used in cell culture and as an ampholytic separator to create a pH gradient in isoelectric focusing. It is used as a non-coordinating buffer in solutions with metal ions because it is not likely to form a complex with metal ions.

The buffer is able to form free radicals so it is not suitable for redox reactions. It is reported that oxidative DNA damage can be induced by free radicals generated from HEPES buffer in the presence of Au(III).

In addition, it is demonstrated that HEPES in RPMI medium potently increases hydrogen peroxide and cytotoxic products during exposure to medium to visible light.

 

Applications

• Buffer

• Cell culture

• BCA assay

 

Specifications

• Endotoxin (0.2% Solution): ≤ 0.25 EU/mL
• Heavy Metals (as Pb): ≤ 0.001 %
• Iron (Fe): ≤ 0.0005 %
• Loss on Drying: ≤ 1.0 %
• pH (5% in water): 5.0 - 6.5
• pKa @ 20°C: 7.35 - 7.70
• Residue on Ignition: ≤ 0.1 %
• Sulfate: ≤ 0.05 %
• UV Absorbance (1M@260nm): ≤ 0.05
• UV Absorbance (1M@280nm): ≤ 0.05

 

 References

• “Oxidative DNA damage induced by HEPES (2-[4-(2-hydroxyethyl)-1-piperazinyl]ethanesulfonic acid) buffer in the presence of Au(III)”

• “Analysis of the cytotoxic effects of light-exposed hepes-containing culture medium”

 

Not for human therapeutic use or for medicinal purposes. For research applications only.


More Information
Alternate Name/Synonyms
HEPES; Monosodium Salt HEPES
SKU
H-2431
CAS #
7365-45-9
Chemical Name
2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid
Chemical Formula
C8H18N2O4S
Molecular Weight
238.3 Da
Appearance
White crystalline powder
Purity
≥99.5% (titration, dried basis)
Solubility
Water: clear and colorless solution
Melting Point
234 °C
Storage Temp
Room temperature
THERAPEUTIC AREA
Not Applicable
USE
HEPES (4-(2-Hydroxyethyl)piperazine-1-ethane-sulfonic acid) has been described as one of the best all-purpose buffers available for use in biological research. The molecule is zwitterionic at most biological pHs and is most effective as a buffer at pH 6.0 to 8.5. May be used in isoelectric focusing applications and as a buffer in the quantitative and selective measurement of antigen-antibody reactions. HEPES is reportedly superior to NaHCO3 in controlling pH in tissue and organ culture. Not recommended for use with the Folin-Ciocalteu protein assay.
MDL NUMBER
MFCD00006158
CHEMACX
X1001076-9
INCHI
JKMHFZQWWAIEOD-UHFFFAOYSA-N
SMILES
C1CN(CCN1CCO)CCS(=O)(=O)O
Peptide sequence
Not Applicable
RTECS
TL6809000
UN #'S
Not Applicable
PACKING GROUP
Not Applicable
HAZARD CLASS
Not Applicable
Handling
Use only in area provided with appropriate exhaust ventilation. Keep away from heat and source of ignition. Empty containers pose a fire risk, evaporate residue under fume hood. Ground all equipment containing material. Do not breathe dust.
msds 1
H-2431, HEPES, free acid, SDS, diamond format.pdf
Certificate of Analysis 1
H-2431, K1550.pdf
Certificate of Analysis 2
H-2431, J1048.pdf
Certificate of Analysis 3
H-2431, F1234.pdf
GHS PICTOGRAMS
Handling Use only in area provided with appropriate exhaust ventilation. Keep away from heat and source of ignition. Empty containers pose a fire risk, evaporate residue under fume hood. Ground all equipment containing material. Do not breathe dust.
UN #'S Not Applicable
Packing Group Not Applicable
Citations Stendomycin selectively inhibits TIM23-dependent mitochondrial protein import
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