HEPES, Free Acid
SKU
H-2431
- CAS:7365-45-9
- Formula:C8H18N2O4S
- MW:238.3 Da
- Appearance:White crystalline powder
- Purity:≥99.5% (titration, dried basis)
SDS
CofA
Description
ZwiterionicN-substituted Aminosulfonic Acid Buffer useful in cell culture media formulations. This buffer is capable of forming radicals and is therefore not suitable for redox reactions.
Applications
• used in isoelectric focusing applications
• as a buffer in the quantitative and selective measurement of antigen-antibody reactions
• not recommended for use with the Folin-Ciocalteu protein assay
Specifications
• Endotoxin (0.2% Solution): ≤ 0.25 EU/mL
• Heavy Metals (as Pb): ≤ 0.001 %
• Iron (Fe): ≤ 0.0005 %
• Loss on Drying: ≤ 1.0 %
• pH (5% in water): 5.0 - 6.5
• pKa @ 20°C: 7.35 - 7.70
• Residue on Ignition: ≤ 0.1 %
• Sulfate: ≤ 0.05 %
• UV Absorbance (1M@260nm): ≤ 0.05
• UV Absorbance (1M@280nm): ≤ 0.05
More Information
Alternate Name/Synonyms
HEPES; Monosodium Salt HEPES
SKU
H-2431
CAS #
7365-45-9
Chemical Name
2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid
Chemical Formula
C8H18N2O4S
Molecular Weight
238.3 Da
Appearance
White crystalline powder
Purity
≥99.5% (titration, dried basis)
Solubility
Water: clear and colorless solution
Melting Point
234 °C
Preparation
N/A
Storage Temp
Room temperature
THERAPEUTIC AREA
Not Applicable
USE
HEPES (4-(2-Hydroxyethyl)piperazine-1-ethane-sulfonic acid) has been described as one of the best all-purpose buffers available for use in biological research. The molecule is zwitterionic at most biological pHs and is most effective as a buffer at pH 6.0 to 8.5. May be used in isoelectric focusing applications and as a buffer in the quantitative and selective measurement of antigen-antibody reactions. HEPES is reportedly superior to NaHCO3 in controlling pH in tissue and organ culture. Not recommended for use with the Folin-Ciocalteu protein assay.
Merck Index
N/A
MDL NUMBER
MFCD00006158
CHEMACX
X1001076-9
INCHI
JKMHFZQWWAIEOD-UHFFFAOYSA-N
SMILES
C1CN(CCN1CCO)CCS(=O)(=O)O
Peptide sequence
Not Applicable
RTECS
TL6809000
UN #'S
Not Applicable
PACKING GROUP
Not Applicable
HAZARD CLASS
Not Applicable
Handling
Use only in area provided with appropriate exhaust ventilation. Keep away from heat and source of ignition. Empty containers pose a fire risk, evaporate residue under fume hood. Ground all equipment containing material. Do not breathe dust.
Certificate of Analysis 1
Certificate of Analysis 2
Certificate of Analysis 3
| GHS PICTOGRAMS |
|
|---|---|
| Handling | Use only in area provided with appropriate exhaust ventilation. Keep away from heat and source of ignition. Empty containers pose a fire risk, evaporate residue under fume hood. Ground all equipment containing material. Do not breathe dust. |
| UN #'S | Not Applicable |
| Packing Group | Not Applicable |
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