HEPES is a zwitterionic N-substituted aminosulfornic acid buffer used in cell culture and as an ampholytic separator to create a pH gradient in isoelectric focusing. It is used as a non-coordinating buffer in solutions with metal ions because it is not likely to form a complex with metal ions.
The buffer is able to form free radicals so it is not suitable for redox reactions. It is reported that oxidative DNA damage can be induced by free radicals generated from HEPES buffer in the presence of Au(III).
In addition, it is demonstrated that HEPES in RPMI medium potently increases hydrogen peroxide and cytotoxic products during exposure to medium to visible light.
• Cell culture
• BCA assay
• Endotoxin (0.2% Solution): ≤ 0.25 EU/mL
• Heavy Metals (as Pb): ≤ 0.001 %
• Iron (Fe): ≤ 0.0005 %
• Loss on Drying: ≤ 1.0 %
• pH (5% in water): 5.0 - 6.5
• pKa @ 20°C: 7.35 - 7.70
• Residue on Ignition: ≤ 0.1 %
• Sulfate: ≤ 0.05 %
• UV Absorbance (1M@260nm): ≤ 0.05
• UV Absorbance (1M@280nm): ≤ 0.05
Not for human therapeutic use or for medicinal purposes. For research applications only.
|Handling||Use only in area provided with appropriate exhaust ventilation. Keep away from heat and source of ignition. Empty containers pose a fire risk, evaporate residue under fume hood. Ground all equipment containing material. Do not breathe dust.|
|UN #'S||Not Applicable|
|Packing Group||Not Applicable|