Turbo3C (HRV 3C) Protease (known as human rhinovirus 3C protease) is a recombinant cysteine protease. It cleaves between Gln and Gly in Leu-Glu-Val-Leu-Phe-Gln-Gly-Pro, commonly referred to as the PreScission Site. HRV 3C Protease is a 47 kDa protein with robust activity at 4°C. It also has both GST and His tags, which can be removed by either Ni-chelating or Glutathione (GSH) resin.
• Used as a therapeutic implication because of its unique protein structure • Used to achieve high cleavage efficiency without non-specific cleavage of target proteins • Used for the biochemical and structural characterization conducted on HRV 3C protease along with the development of 3C protease inhibitors
HRV 3C Protease, Cysteine Protease, PreScission Site
It is recommended to test the HRV3C Protease cleavage with protease-to-target protein ratio of 1:100 (w/w) in the buffer suitable for the target protein at 4°C overnight, with the target protein concentration at or below 1 mg/ml. Under this condition, > 9
Store at -20°C. HRV3C is stable at room temperature for up to 2 weeks.
Human rhinovirus 3C protease (HRV3C Protease) is a cysteine protease that recognizes the cleavage site of Leu-Glu-Val-Leu-Phe-Gln-Gly-Pro, commonly referred to as the PreScission Site. It cleaves between Gln and Gly. The recombinant Turbo3C Protease is a restriction grade HRV3C protease that has robust activity at 4oC and high specific activity. Turbo3C Protease is a 47 kDa protein with both GST and His tags so it can be removed by either Ni chelating or Glutathione (GSH) resin.