Hygromycin B, Concentrated Solution

SKU
H-1012-MU
  • CAS:31282-04-9
  • Formula:C20H37N3O13
  • MW:527.5 Da
  • Appearance:Light amber colored solution
  • Purity:≥90% (HPLC)

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Starting at $101.61

Product Name Qty
Hygromycin B, Concentrated Solution
1 MU
$101.61
Hygromycin B, Concentrated Solution
5x1 MU
$400.93

Description

Hygromycin B is an antibiotic that is known to be effective against bacteria, fungi, viruses and assorted eukaryotic pathogens. As an aminoglycoside antibiotic, hygromycin B inhibits ribosome biogenesis by targeting the 30S ribosomal subunit and preventing translation. As a result, the compound suppresses protein synthesis within pathogenic cells.

AG Scientific's Hygromycin B solution is a sterile-filtrate solution in DI Water.

Applications

In clinical practice, hygromycin B can be used to treat bacterial infections. In the laboratory, it is frequently used as a selection antibiotic for pathogens that have developed hygromycin resistance. Research has also shown that the aminoglycoside class of antibiotics can suppress certain mutations, a fact which has been leveraged as a treatment for Duchenne dystrophy.

Antibiotics are often used in clinical in vitro tests known as antimicrobial susceptibility tests or ASTs to determine their efficacy against certain bacterial species. Hygromycin's longstanding application as a gene selection antibiotic, has a powerful new role in genome editing utilizing the CRISPR/CAS9 system.

Additional Reading

• Hygromycin B: Frequently Asked Questions

 

More Information
Alternate Name/Synonyms
Hygrovetine; Hygrovectine; D-streptamine; Antihelmycin
SKU
H-1012-MU
CAS #
31282-04-9
Chemical Name
(3'R,3aS,4S,4'S,5'R,6R,6'R,7S,7aS)-4-[(1R,2S,3R,5S,6R)-3-amino-2,6-dihydroxy-5-(methylamino)cyclohexyl]oxy-6'-[(1S)-1-amino-2-hydroxyethyl]-6-(hydroxymethyl)spiro[4,6,7,7a-tetrahydro-3aH-[1,3]dioxolo[4,5-c]pyran-2,2'-oxane]-3',4',5',7-tetrol
Chemical Formula
C20H37N3O13
Molecular Weight
527.5 Da
Appearance
Light amber colored solution
Purity
≥90% (HPLC)
Solubility
Water
Melting Point
N/A
Preparation
N/A
Storage Temp
Store at +4°C
THERAPEUTIC AREA
Infectious diseases
USE
Hygromycin B is an antibiotic produced by the bacterium Streptomyces hygroscopicus. It is an aminoglycoside that kills bacteria, fungi and higher eukaryotic cells by inhibiting protein synthesis. Hygromycin resistance gene is frequently used as a selectable marker in research on plants. In rice Agrobacterium-mediated transformation system, hygromycin is used at about 30–75 mg L−1, with an average of 50 mg L−1. The use of hygromycin at 50 mg L−1 demonstrated highly toxic to non-transformed calli. Thus, it can be efficiently used to select transformants.
Merck Index
13.4878
MDL NUMBER
MFCD06795479
CHEMACX
X1009739-3
INCHI
InChI=1S/C20H37N3O13/c1-23-7-2-5(21)9(26)15(10(7)27)33-19-17-16(11(28)8(4-25)32-19)35-20(36-17)18(31)13(30)12(29)14(34-20)6(22)3-24/h5-19,23-31H,2-4,21-22H2,1H3/t5-,6+,7+,8-,9+,10-,11+,12-,13-,14-,15-,16+,17+,18-,19+,20?/m1/s1
SMILES
CN[C@H]1C[C@H]([C@@H]([C@H]([C@@H]1O)O[C@H]2[C@@H]3[C@H]([C@H]([C@H](O2)CO)O)OC4(O3)[C@@H]([C@@H]([C@H]([C@H](O4)[C@H](CO)N)O)O)O)O)N
RTECS
WK2130000
UN #'S
UN 3462
PACKING GROUP
I
Handling
Warning.Toxic. May be Carcinogenic. May be Teratogenic. Wear gloves/mask when handling product.
Additional Videos
Certificate of Analysis 1
Certificate of Analysis 2
Certificate of Analysis 3
GHS PICTOGRAMS
Handling Warning.Toxic. May be Carcinogenic. May be Teratogenic. Wear gloves/mask when handling product.
UN #'S UN 3462
Packing Group I
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Recombinant human hyaluronidase Hyal-1: insect cells versus Escherichia coli as expression system and identification of low molecular weight inhibitors
Structure-function analysis of Rgs1 in Magnaporthe oryzae: role of DEP domains in subcellular targeting
Synthesis of non-natural flavanones and dihydrochalcones in metabolically engineered yeast
Protective Vaccine Efficacy of the Complete Form of PPE39 Protein from Mycobacterium tuberculosis Beijing/K Strain in Mice
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The Function of Phosphatidylinositol 4-Kinase III-Beta in Trypanosoma Brucei
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Quinocarmycin analog DX-52-1 inhibits cell migration and targets radixin, disrupting interactions of radixin with actin and CD44
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Production of multivalent protein binders using a self-trimerizing collagen-like peptide scaffold
Label-free analysis of GPCR-stimulation: The critical impact of cell adhesion
DEMETER plant DNA demethylase induces antiviral response by interferon signalling in animal cells
Conservation of tRNA and rRNA 5-methylcytosine in the kingdom Plantae
Fusarium verticillioides chitin synthases CHS5 and CHS7 are required for normal growth and pathogenicity
Host-specific transcriptomic pattern of Trichoderma virens during interaction with maize or tomato roots
Role of the tumor suppressor PTEN in antioxidant responsive element-mediated transcription and associated histone modifications
MprA and DosR coregulate a Mycobacterium tuberculosis virulence operon encoding Rv1813c and Rv1812c
Discordant influence of Blastomyces dermatitidis yeast-phase-specific gene BYS1 on morphogenesis and virulence
CaMKIIalphaB mediates a survival response in retinal ganglion cells subjected to a glutamate stimulus
Rv2744c Is a PspA Ortholog That Regulates Lipid Droplet Homeostasis and Nonreplicating Persistence in Mycobacterium tuberculosis
The MprB extracytoplasmic domain negatively regulates activation of the Mycobacterium tuberculosis MprAB two-component system
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Cationic Liposomes: A Flexible Vaccine Delivery System for Physicochemically Diverse Antigenic Peptides
Development and application of a green fluorescent protein sentinel system for identification of RNA interference in Blastomyces dermatitidis illuminates the role of septin in morphogenesis and sporulation
Luciferase reporter gene assay on human, murine and rat histamine H4 receptor orthologs: correlations and discrepancies between distal and proximal readouts
The HtrA-like serine protease PepD interacts with and modulates the Mycobacterium tuberculosis 35-kDa antigen outer envelope protein
Blastomyces dermatitidis septins CDC3, CDC10, and CDC12 impact the morphology of yeast and hyphae, but are not required for the phase transition
Expression of the bacteriophage T4 lysozyme gene in tall fescue confers resistance to gray leaf spot and brown patch diseases
Development and evaluation of an M2-293FT cell-based flow cytometric assay for quantification of antibody response to native form of matrix protein 2 of influenza A viruses
Agrobacterium tumefaciens-mediated transformation of big bluestem (Andropogon gerardii Vitman)
Transformation of Candida albicans with a synthetic hygromycin B resistance gene
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Characterization Of A Novel Acetyltransferase Found Only In Pathogenic Strains Of Mycobacterium tuberculosis
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Characterization of mammalian hyaluronidase-2 activity and identification of inhibitors of Streptococcal hyaluronan lyase
Baculoviral transduction facilitates TALEN-mediated targeted transgene integration and Cre/LoxP cassette exchange in human-induced pluripotent stem cells
Efficient recombinase-mediated cassette exchange at the AAVS1 locus in human embryonic stem cells using baculoviral vectors
Zinc finger nuclease-expressing baculoviral vectors mediate targeted genome integration of reprogramming factor genes to facilitate the generation of human induced pluripotent stem cells
Targeted transgene insertion into the AAVS1 locus driven by baculoviral vector-mediated zinc finger nuclease expression in human-induced pluripotent stem cells
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Inducible expression systems for mycobacteria
A simplified protocol for genetic transformation of switchgrass (Panicum virgatum L.)
Enhancement of androgen transcriptional activation assay based on genome edited glucocorticoid knock out human prostate cancer cell line
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