Hygromycin B in DI Water

  • CAS:31282-04-9
  • Formula:C20H37N3O13
  • MW:527.5 Da
  • Appearance:Clear and yellowish solution
  • Purity:≥90% (HPLC)

Starting at $106.02

Product Name Qty
Hygromycin B in DI Water
1x1 g (1x10 mL)
Hygromycin B in DI Water
5x200 mg (5x2 mL)
Hygromycin B in DI Water
5x1 g (5x10 mL)

* The Concentration of Hygromycin B in DI water is 100mg/mL. AG Scientific's Hygromycin B solution is a a sterile filterate solution.


Hygromycin B is an aminoglycoside antibiotic effective against bacteria, fungi, and eukaryotic cells that are isolated from Streptomyces hygroscopicus.  It is composed of amino groups attached to glycosides, that bind the 30s ribosomal subunits. This action causes the mRNA sequence to be misread, resulting in protein synthesis inhibition. Hygromycin B is also an antiviral agent, which works as a standard section antibiotic in gene experiments.


• Used to study protein synthesis
• Used in clinical in vitro tests known as antimicrobial susceptibility tests or ASTs to determine their efficacy against certain bacterial species
• Used as a gene selection antibiotic has a powerful new role in Genome editing utilizing the CRISPR/Cas9 system
• Used to inhibit translocation of mRNA and tRNAs on the ribosome in both bacteria and eukaryotes

Additional Reading

• Hygromycin B: Frequently Asked Questions


Not for human therapeutic use or for medicinal purposes. For research applications only.

More Information
Alternate Name/Synonyms
Hygrovetine; Hygrovectine; D-streptamine; Antihelmycin
Chemical Name
Chemical Formula
Molecular Weight
527.5 Da
Clear and yellowish solution
≥90% (HPLC)
Storage Temp
Store at +4°C
Infectious diseases
Hygromycin B is an antibiotic produced by the bacterium Streptomyces hygroscopicus. It is an aminoglycoside that kills bacteria, fungi and higher eukaryotic cells by inhibiting protein synthesis. It is commonly used to study protein synthesis. It works as a standard selection antibiotic in gene experiments, especially for selection of hygromycin resistance gene transformed cells. Aside from its efficacy against bacteria, fungi, and higher eukaryotic cells, hygromycin B is also an antiviral agent inhibiting translation upon selectively entering cells made permeable due to viral infection.
Merck Index
UN #'S
UN 3462
Warning: Toxic! May be carcinogenic, may be teratogenic. Use only in area with appropriate exhaust ventilation. Protect from light!
Additional Videos
Certificate of Analysis 1
Certificate of Analysis 2
Certificate of Analysis 3
HandlingWarning: Toxic! May be carcinogenic, may be teratogenic. Use only in area with appropriate exhaust ventilation. Protect from light!
UN #'SUN 3462
Packing GroupI
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