Hygromycin B, High Purity In PBS Buffer

SKU
H-1012-PBS
  • CAS:31282-04-9
  • Formula:C20H37N3O13
  • MW:527.5 Da
  • Appearance:Clear, yellowish solution
  • Purity:≥90% (HPLC)

4

0

0

Product Name Qty
Hygromycin B, High Purity In PBS Buffer
10x100mg (10x2ml)
$112.75
Hygromycin B, High Purity In PBS Buffer
1x1g (1x20ml)
$101.61
Hygromycin B, High Purity In PBS Buffer
5x1g (5x20ml)
$400.93

* The concentration of Hygromycin B in PBS buffer is 50 mg/ml 

Hygromycin B is an aminoglycoside antibiotic isolated from Streptomyces hygroscopicus. It is commonly used to study protein synthesis. Aside from its efficacy against bacteria, fungi, and higher eukaryotic cells, hygromycin B is also an antiviral agent inhibiting translation upon selectively entering cells made permeable due to viral infection. It works as a standard selection antibiotic in gene experiments, especially for selection of hygromycin resistance gene transformed cells.

Aminoglycoside antibiotics are composed of amino groups attached to glycosides. They bind the 30s ribosomal subunit, causing misreading of the mRNA sequence and inhibition of translocation. Consequently, protein synthesis is inhibited.

Antibiotics are often used in clinical in vitro tests known as antimicrobial susceptibility tests or ASTs to determine their efficacy against certain bacterial species. Hygromycin's longstanding application as a gene selection antibiotic, has a powerful new role in genome editing utilizing the CRISPR/CAS9 system.

 

Additional Reading

• Hygromycin B: Frequently Asked Questions

 

* ATTENTION! International shipping of this material can be subject to an additional hazardous shipping fee. An AG Scientific customer service representative will contact you to confirm cost prior to shipping your international order.  

 

More Information
Alternate Name/Synonyms
Hygrovetine; Hygrovectine; D-streptamine
SKU
H-1012-PBS
CAS #
31282-04-9
Chemical Name
(3'R,3aS,4S,4'S,5'R,6R,6'R,7S,7aS)-4-[(1R,2S,3R,5S,6R)-3-amino-2,6-dihydroxy-5-(methylamino)cyclohexyl]oxy-6'-[(1S)-1-amino-2-hydroxyethyl]-6-(hydroxymethyl)spiro[4,6,7,7a-tetrahydro-3aH-[1,3]dioxolo[4,5-c]pyran-2,2'-oxane]-3',4',5',7-tetrol
Chemical Formula
C20H37N3O13
Molecular Weight
527.5 Da
Appearance
Clear, yellowish solution
Purity
≥90% (HPLC)
Solubility
Soluble in water
Melting Point
N/A
Preparation
Working solutions (<2mg/ml) are stable for ~1 month at +4°C.
Storage Temp
Store at +4°C
THERAPEUTIC AREA
Infectious diseases
USE
Hygromycin B is an aminoglycoside antibiotic that kills bacteria and fungi.
Merck Index
13.4878
MDL NUMBER
MFCD06795479
CHEMACX
X1009739-3
INCHI
InChI=1S/C20H37N3O13/c1-23-7-2-5(21)9(26)15(10(7)27)33-19-17-16(11(28)8(4-25)32-19)35-20(36-17)18(31)13(30)12(29)14(34-20)6(22)3-24/h5-19,23-31H,2-4,21-22H2,1H3/t5-,6+,7+,8-,9+,10-,11+,12-,13-,14-,15-,16+,17+,18-,19+,20?/m1/s1
SMILES
CN[C@H]1C[C@H]([C@@H]([C@H]([C@@H]1O)O[C@H]2[C@@H]3[C@H]([C@H]([C@H](O2)CO)O)OC4(O3)[C@@H]([C@@H]([C@H]([C@H](O4)[C@H](CO)N)O)O)O)O)N
RTECS
WK2130000
PACKING GROUP
I
Handling
Protect from light
Citations
MOLECULAR PATHOGENESIS: MprA and DosR Coregulate a Mycobacterium tuberculosis Virulence Operon Encoding Rv1813c and Rv1812c Daniel J. Bretl, Hongjun He, Crystalla Demetriadou, Mark J. White, Renee M. Penoske, Nita H. Salzman, and Thomas C. Zahrt Infect. Immun., Sep 2012; 80: 3018 - 3033.
Citations_Hyperlink
http://iai.asm.org/content/80/9/3018.full.pdf+html
Additional Videos
Certificate of Analysis 1
Certificate of Analysis 2
Certificate of Analysis 3
GHS PICTOGRAMS
Handling Protect from light
Packing Group I
We found other products you might like!
To Top