Hygromycin B Solid, High Purity, Cell Culture Tested

SKU
H-1012-SOLID
  • CAS:31282-04-9
  • Formula:C20H37N3O13
  • MW:527.5 Da
  • Appearance:Cream colored powder
  • Purity:≥90% (HPLC)

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Product Name Qty
Hygromycin B, Solid, High Purity, Cell Culture Tested
5x1 g
$400.93
Hygromycin B, Solid, High Purity, Cell Culture Tested
1 g
$101.61
Hygromycin B, Solid, High Purity, Cell Culture Tested
10 g
$723.66

*IF >1GM HAZARDOUS BOX FEE APPLIED*

Hygromycin B is an aminoglycoside antibiotic isolated from Streptomyces hygroscopicus. It is commonly used to study protein synthesis. Aside from its efficacy against bacteria, fungi, and higher eukaryotic cells, hygromycin B is also an antiviral agent inhibiting translation upon selectively entering cells made permeable due to viral infection. It works as a standard selection antibiotic in gene experiments, especially for the selection of hygromycin resistance gene transformed cells. Aminoglycoside antibiotics are composed of amino groups attached to glycosides. They bind the 30s ribosomal subunit, causing misreading of the mRNA sequence and inhibition of translocation. Consequently, protein synthesis is inhibited.

Antibiotics are often used in clinical in-vitro tests known as antimicrobial susceptibility tests or ASTs to determine their efficacy against certain bacterial species. Hygromycin's longstanding application as a gene selection antibiotic has a powerful new role in genome editing utilizing the CRISPR/CAS9 system.

Additional Reading

• Hygromycin B: Frequently Asked Questions

 

 * ATTENTION! International shipping of this material can be subject to an additional hazardous shipping fee. An AG Scientific customer service representative will contact you to confirm cost prior to shipping your international order.  

 

More Information
Alternate Name/Synonyms
Hygrovetine; Hygrovectine; D-streptamine
SKU
H-1012-SOLID
CAS #
31282-04-9
Chemical Name
(3'R,3aS,4S,4'S,5'R,6R,6'R,7S,7aS)-4-[(1R,2S,3R,5S,6R)-3-amino-2,6-dihydroxy-5-(methylamino)cyclohexyl]oxy-6'-[(1S)-1-amino-2-hydroxyethyl]-6-(hydroxymethyl)spiro[4,6,7,7a-tetrahydro-3aH-[1,3]dioxolo[4,5-c]pyran-2,2'-oxane]-3',4',5',7-tetrol
Chemical Formula
C20H37N3O13
Molecular Weight
527.5 Da
Appearance
Cream colored powder
Purity
≥90% (HPLC)
Solubility
Water (50 mg/mL): clear, light-tan solution
Melting Point
N/A
Preparation
Working solutions (<2mg/ml) are stable for ~1 month at +4°C. Hygromycin B is weakly basic.
Storage Temp
Store at +4°C
THERAPEUTIC AREA
Infectious diseases
USE
Hygromycin B is an aminoglycoside antibiotic that kills bacteria and fungi.
Merck Index
13.4878
MDL NUMBER
MFCD06795479
CHEMACX
X1009739-3
INCHI
InChI=1S/C20H37N3O13/c1-23-7-2-5(21)9(26)15(10(7)27)33-19-17-16(11(28)8(4-25)32-19)35-20(36-17)18(31)13(30)12(29)14(34-20)6(22)3-24/h5-19,23-31H,2-4,21-22H2,1H3/t5-,6+,7+,8-,9+,10-,11+,12-,13-,14-,15-,16+,17+,18-,19+,20?/m1/s1
SMILES
CN[C@H]1C[C@H]([C@@H]([C@H]([C@@H]1O)O[C@H]2[C@@H]3[C@H]([C@H]([C@H](O2)CO)O)OC4(O3)[C@@H]([C@@H]([C@H]([C@H](O4)[C@H](CO)N)O)O)O)O)N
RTECS
WK2130000
UN #'S
UN 3462
PACKING GROUP
I
Handling
Warning.Toxic! May be carcinogenic. May be teratogenic. Protect from moisture!
Special Shipping
Yes
Citations
BIOSYNTHESIS AND BIODEGRADATION: Ergosterol content specifies targeting of tail-anchored proteins to mitochondrial outer membranes Katrin Krumpe, Idan Frumkin, Yonatan Herzig, Nitzan Rimon, Cagakan Özbalci, Britta Brügger, Doron Rapaport, and Maya Schuldiner Mol. Biol. Cell, Oct 2012; 23: 3927 - 3935.
Citations_Hyperlink
http://www.molbiolcell.org/content/23/20/3927.full.pdf+html
Additional Videos
Certificate of Analysis 1
Certificate of Analysis 2
Certificate of Analysis 3
GHS PICTOGRAMS
Handling Warning.Toxic! May be carcinogenic. May be teratogenic. Protect from moisture!
UN #'S UN 3462
Packing Group I
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TAK1 MAPK kinase kinase mediates transforming growth factor-beta signaling by targeting SnoN oncoprotein for degradation
Role of glutathione in the oxidative stress response in the fungal pathogen Candida glabrata
Sequence analysis of rice rubi3 promoter gene expression cassettes for improved transgene expression
Expression enhancement of a rice polyubiquitin gene promoter
PepD participates in the mycobacterial stress response mediated through MprAB and SigE
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ER-alpha-cDNA as part of a bicistronic transcript gives rise to high frequency, long term, receptor expressing cell clones
Fluorescence Reporter-Based Genome-Wide RNA Interference Screening to Identify Alternative Splicing Regulators
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