Hygromycin B Solid, High Purity, Cell Culture Tested

  • CAS:31282-04-9
  • Formula:C20H37N3O13
  • MW:527.5 Da
  • Appearance:Cream colored powder
  • Purity:≥90% (HPLC)

Starting at $106.02

Product Name Qty
Hygromycin B, Solid, High Purity, Cell Culture Tested
1 g
Hygromycin B, Solid, High Purity, Cell Culture Tested
5x1 g
Hygromycin B, Solid, High Purity, Cell Culture Tested
10 g



Hygromycin B is an aminoglycoside antibiotic isolated from Streptomyces hygroscopicus. It is commonly used to study protein synthesis. Aside from its efficacy against bacteria, fungi, and higher eukaryotic cells, hygromycin B is also an antiviral agent inhibiting translation upon selectively entering cells made permeable due to viral infection. It works as a standard selection antibiotic in gene experiments, especially for the selection of hygromycin resistance gene transformed cells. Aminoglycoside antibiotics are composed of amino groups attached to glycosides. They bind the 30s ribosomal subunit, causing misreading of the mRNA sequence and inhibition of translocation. Consequently, protein synthesis is inhibited.

Antibiotics are often used in clinical in-vitro tests known as antimicrobial susceptibility tests or ASTs to determine their efficacy against certain bacterial species. Hygromycin's longstanding application as a gene selection antibiotic has a powerful new role in genome editing utilizing the CRISPR/CAS9 system.


Additional Reading

• Hygromycin B: Frequently Asked Questions


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Not for human therapeutic use or for medicinal purposes. For research applications only.


More Information
Alternate Name/Synonyms
Hygrovetine; Hygrovectine; D-streptamine
Chemical Name
Chemical Formula
Molecular Weight
527.5 Da
Cream colored powder
≥90% (HPLC)
Water (50 mg/mL): clear, light-tan solution
Working solutions (<2mg/ml) are stable for ~1 month at +4°C. Hygromycin B is weakly basic.
Storage Temp
Store at +4°C
Infectious diseases
Hygromycin B is an aminoglycoside antibiotic that kills bacteria and fungi.
Merck Index
UN #'S
UN 3462
Warning.Toxic! May be carcinogenic. May be teratogenic. Protect from moisture!
Special Shipping
BIOSYNTHESIS AND BIODEGRADATION: Ergosterol content specifies targeting of tail-anchored proteins to mitochondrial outer membranes Katrin Krumpe, Idan Frumkin, Yonatan Herzig, Nitzan Rimon, Cagakan Özbalci, Britta Brügger, Doron Rapaport, and Maya Schuldiner Mol. Biol. Cell, Oct 2012; 23: 3927 - 3935.
Additional Videos
Certificate of Analysis 1
Certificate of Analysis 2
Certificate of Analysis 3
HandlingWarning.Toxic! May be carcinogenic. May be teratogenic. Protect from moisture!
UN #'SUN 3462
Packing GroupI
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