IPTG known as Isopropyl-β-D-Thiogalactopyranoside functions as an inducer of galactosidase activity by binding to and inhibiting the repressor.
It is utilized for the induction of expression from the lac promoter and derivates. IPTG is also used to differentiate recombinants from non-recombinants in cloning strategies using vectors containing the Z or Z peptide gene.
• Used in cloning procedures that require induction of β-galactosidase activity
• Used in cellular systems to study gene activity
• Used in conjunction with X-Gal or Bluo-Gal in blue-white selection of recombinant bacterial colonies that induce expression of the lac operon in Escherichia coli
Additional Reading from AG Scientific
Not for human therapeutic use or for medicinal purposes. For research applications only.
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