Kanamycin is an aminoglycosidic antibiotic that inhibits growth of gram-positive, gram-negative bacteria and mycoplasmas in cell culture. Kanamycin or kanamycin A is a major component of the antibiotic complex produced by certain strains of Streptomyces kanamyceticus. Kanamycin is supplied in two forms, a crystalline monosulfate monohydrate and a salt with a higher sulfate content. Kanamycin sulfate, which is sometimes referred to as “kanamycin bisulfate,” is obtained by adding sulfuric acid to a solution of the monosulfate and drying using a suitable procedure. Kanamycin sulfate binds to 30S ribosomes causing inhibition of bacterial protein synthesis.
• Lab Application - used as a resistance marker to grow plasmids of interest in competent bacterial cells • Therapeutic Use - used as a second-line anti-tuberculosis drug
Kanamycin Sulfate Solution is used as a selection agent for cells transformed with kanamycin B resistance gene. It is also used as a culture media additive for the isolation of group D streptococci on Kanamycin Esculin Azide Agar and for selection of transformed plant cells containing the neomycin phosphotransferase on a kanamycin-medium. It can also be used as a selection agent for cells transformed with kanamycin B resistance gene. Indications of kanamycin is short term treatment of bacterial infections caused by the following pathogens: E. coli, Proteus species (both indole-positive and indole-negative), Enterobacter aerogenes, Klebsiella pneumoniae, Serratia marcescens, and Acinetobacter species.