MOPS, Free Acid
- MW:209.3 Da
- Appearance:White crystalline power
MOPS, also known as 3-(N-morpholino)propanesulfonic acid, is one of Good’s buffers. It is N-substituted amino sulfonic acid with a morpholinic ring and has zwitterionic and inert properties. MOPS buffer is broadly used in biological and biochemical studies. It can be mixed with MOPS sodium salt to attain desired pH.
MOPS is recommended as an agent for non-coordinating buffers in solutions with metal ions because it hardly forms a complex with most metal ions such as Cu, Ni, Mn, Zn and Co ions.
The Good's buffer interferes with the Folin protein assay and partially decomposes when autoclaved in the presence of glucose. MOPS can be used as a Good’s buffer because it has low UV absorptivity, minimal reactivity, stable pH and is soluble in water.
• Heavy Metals: <5 ppm
• Iron (Fe): ≤ 5 ppm
• Endotoxin : ≤ 0.5 EU/mg
• pKa at 20°C: 7.20 ± 0.2
• pH range: 6.5-7.9
• Absorbance at 250 nm, 1M solution in water: ≤ 0.1
• Absorbance at 260 nm: ≤ 0.2
• Absorbance at 280 nm: ≤ 0.2
• Loss on Drying (105 oC): ≤ 1.0 %
• Residue on Ignition: ≤ 0.2 %
• Used as a buffer in cell culture media
• Used as a running buffer in electrophoresis and chromatography
Not for human therapeutic use or for medicinal purposes. For research applications only.
|Handling||Wear personal protective equipment. Ensure adequate ventilation. Avoid dust formation. Do not breathe dust. Do not get in eyes, on skin, or on clothing.|