MOPS is a zwitterionic buffer used as a running buffer for denaturing gel electrophoresis and as a buffering agent in many biological and biochemical applications. MOPS interferes with the Folin protein assay and partially decomposes when autoclaved in the presence of glucose. MOPS can be used as a Good’s buffer because it has low UV absorptivity, minimal reactivity, stable pH and is soluble in water.
• Heavy Metals: <5 ppm • Iron (Fe): ≤ 5 ppm • Endotoxin : ≤ 0.5 EU/mg • pKa at 20°C: 7.20 ± 0.2 • pH range: 6.5-7.9 • Absorbance at 250 nm, 1M solution in water: ≤ 0.1 • Absorbance at 260 nm: ≤ 0.2 • Absorbance at 280 nm: ≤ 0.2 • Loss on Drying (105 oC): ≤ 1.0 % • Residue on Ignition: ≤ 0.2 %
MOPS, Free Acid may be used in the preparation of MOPS buffer. MOPS is a zwitterionic buffer primarily used as a running buffer for denaturing gel electrophoresis. The pKa of MOPS (7.2) is closer to physiological pH than that of MES (6.1), and thus MOPS may be more suitable as a physiologically relevant buffer. MOPS interferes with the Folin protein assay, and partially decomposes when autoclaved in the presence of glucose. Usage above 20 mM in mammalian cell culture work is not recommended.
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