PMSF (Phenylmethylsulfonyl fluoride)

SKU
P-2401
  • CAS:329-98-6
  • Formula:C7H7FO2S
  • MW:174.2 Da
  • Appearance:White fine needles/crystalline powder
  • Purity:≥98.5 %

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Product Name Qty
PMSF
25 g
$194.00
PMSF
100 g
$695.00
PMSF
5 g
$79.00

Description and Applications

Phenylmethylsulfonyl fluoride (PMSF) is an important serine protease inhibitor commonly utilized by biochemists and researchers, phenylmethylsulfonyl fluoride (PMSF) is often used to prepare cell lysates, helping to preserve critical samples.

Acting as a non-specific inhibitor, PMSF is often used to protect samples against the digestive functions of proteases, such as trypsin, chymotrypsin, and thrombin, as well as other enzymes. It is also used for protein purification when preparing protein extracts taken from cells and tissues.

By deactivating the serine hydroxyl group of any enzyme that contains this amino acid, PMSF prevents the degradation of proteins of interest. Based on its short half-life, PMSF must be added during each purification step.

More Information
Alternate Name/Synonyms
Phenylmethylsulfonyl fluoride; Benzylsulfonyl fluoride
SKU
P-2401
CAS #
329-98-6
Chemical Name
Phenylmethanesulfonyl fluoride
Chemical Formula
C7H7FO2S
Molecular Weight
174.2 Da
Appearance
White fine needles/crystalline powder
Purity
≥98.5 %
Solubility
Soluble in 10% pure ethanol, chloroform and diethyl ether. Clear, colorless solution in chloroform and diethyl ether.
Stability
PMSF has limited water solubility, it must be dissolved in a small amount of solvent, such as ethanol.
Melting Point
93.8°C
Preparation
Dissolve PMSF in isopropanol at 1.74 mg/mL (10 mM); store in aliquots at -20°C
Storage Temp
4°C, desiccated
THERAPEUTIC AREA
Analgesic
USE
PMSF inhibits serine proteases such as trypsin and chymotrypsin. Also inhibits cysteine proteases (reversible by reduced thiols) and mammalian acetylcholinesterase. It has been used in following applications; cell fractionation, a supplement in nuclear protein extraction, inhibitor of cholesterol esterase (CE) and pseudocholinesterase (PCE) and for the collection of blood prior to centrifugation to quantify plasma ANP levels.
Merck Index
N/A
MDL NUMBER
MFCD00007424
CHEMACX
X1032413-5
INCHI
InChI=1S/C7H7FO2S/c8-11(9,10)6-7-4-2-1-3-5-7/h1-5H,6H2
SMILES
c1ccc(cc1)CS(=O)(=O)F
Peptide sequence
Not Applicable
RTECS
XT8040000
UN #'S
UN 2928
PACKING GROUP
II
HAZARD CLASS
Not Applicable
Handling
Use only in area provided with appropriate exhaust ventilation. Keep away from heat and source of ignition. Empty containers pose a fire risk, evaporate residue under fume hood. Ground all equipment containing material. Do not breathe dust.
msds 1
/media/upload/pdf/P-2401, PMSF, SDS, diamond format.pdf
Certificate of Analysis 1
/media/upload/pdf/P-2401, H1328C.pdf
Certificate of Analysis 2
/media/upload/pdf/P-2401, H1328B.pdf
Certificate of Analysis 3
/media/upload/pdf/P-2401, H1328A.pdf
GHS PICTOGRAMS
Handling Use only in area provided with appropriate exhaust ventilation. Keep away from heat and source of ignition. Empty containers pose a fire risk, evaporate residue under fume hood. Ground all equipment containing material. Do not breathe dust.
UN #'S UN 2928
Packing Group II
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