Description and Applications
Phenylmethylsulfonyl fluoride (PMSF) is an important serine protease inhibitor commonly utilized by biochemists and researchers, phenylmethylsulfonyl fluoride (PMSF) is often used to prepare cell lysates, helping to preserve critical samples.
Acting as a non-specific inhibitor, PMSF is often used to protect samples against the digestive functions of proteases, such as trypsin, chymotrypsin, and thrombin, as well as other enzymes. It is also used for protein purification when preparing protein extracts taken from cells and tissues.
By deactivating the serine hydroxyl group of any enzyme that contains this amino acid, PMSF prevents the degradation of proteins of interest. Based on its short half-life, PMSF must be added during each purification step.
Additional Reading: PMSF Serine Protease Inhibitor Applications
|Handling||Use only in area provided with appropriate exhaust ventilation. Keep away from heat and source of ignition. Empty containers pose a fire risk, evaporate residue under fume hood. Ground all equipment containing material. Do not breathe dust.|
|UN #'S||UN 2928|