Benzamidine Agarose 6B is p-aminobenzamidine covalently attached to Agarose 6B by the epoxy coupling method. p-Aminobenzamidine (PAB), is an inhibitor of trypsin-like serine protease. Trypsin and trypsin-like serine proteases bind to Benzamidine-Agarose 6B and can thus be used for purification and/or removal of these substances.
Benzamidine-Agarose 6B can be used for purifying serine proteases such as trypsin, bovine thrombin, urokinase, human enterokinase, acrosin, native plasminogen, kallikrein, prekallikrein, collagenase and clostripain. For recombinant purification, Benzamidine-Agarose 6B can be used for removal of serine proteases such as thrombin and enterokinase after cleavage of purification tags.
Benzamiding-Agarose 6B is tested for its binding capacity for trypsin, but the resin has been used in the purification of a variety of proteins including thrombin, Adenylyl cyclase-activating protease from bovine sperm, Fibrinolytic enzyme from Agkistrodon contortrix, Tryptase from rat skin,
Serine endopeptidase from rat mammary tissue, Glycosylation-enhancing factor (GEF), Plasminogen activator from embryo lung culture, Plasminogen activator from human umbilical vein endothelial cells and Thrombin-like enzyme from Bothrops atrox venom
- Ligand: p-Aminobenzamidine (PAB)
- Matrix: Separopore® 6B (agarose beads, 6%)
- Particle size range: 52 – 165 µm
- Matrix activation: Epoxy activation
- Ligand density: 7 μmole p-aminobenzamidine/ml drained gel
- Binding capacity: > 10mg trypsin/ml drained gel
- Exclusion limit: approximately 2 x 107
- pH stability: Long term: 3 – 11; Short term: 2 – 13
- Chemical stability: Stable to all commonly used aqueous buffers
- Physical stability: Negligible volume variation due to changes in pH or ionic strength
- Storage temp: 4 –8 °C
- Benzamidine-Agarose 6B is supplied as pre-swollen gel in 20% ethanol
Research or further manufacturing use only, not for food or drug use.