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Biotin-Separopore® binds with high affinity to avidin and streptavidin and is used to purify (strept)avidin linked biomolecules such as proteins coupled to strept(avidin). The interaction of biotin with avidin is the strongest known non-covalent interaction between a protein and its ligand (Ka = 1015 M-1). Bond formation is rapid and is unaffected by most extremes of pH, organic solvents and other denaturing agents. Immobilized biotin will release avidin if exposed to 8M guanidine-HCl at pH 1.5 or if boiled in reducing SDS-PAGE sample Buffer. These elution conditions permanently denature the avidin protein. It has been widely used in biochemistry and molecular biology for detecting and labeling proteins. For mild conditions, use 2-Iminobiotin-Separopore® 4B-CL (SKU # 20181056).
- Ligand: Biotin
- Matrix: Separopore® 4B-CL (crosslinked agarose beads, 4%)
- Particle size range: 52 – 165 µm
- Molecular weight range: 6 x 104 – 2 x 107
- Matrix activation: Epoxy
- Matrix attachment: Carboxy (amide linkage through diaminopropane spacer); matrix contains residual amino groups which are charged at physiological pH
- Matrix spacer: 13 atoms
- Ligand density: 2 – 6 μmol / ml drained gel
- Binding capacity: 20 – 30 mg of avidin / ml drained gel
- pH stability: 4 – 9
- Flow specifications: 70 – 140 cm / h
- Supplied as suspension in phosphate buffered saline containing 0.02% sodium azide
Research or further manufacturing use only, not for food or drug use.