Reduced glutathione is covalently linked Agarose 4B Microbeads for use in affinity purification of glutathione-S-transferase (GST) and GST-fused proteins. This product provides a one step purification method and permits rapid, mild and highly selective purifications of proteins containing glutathione binding sequences. Glutathione-Agarose is prepared by covalently coupling glutathione to epoxy-activated 4% crosslinked agarose beads to form a stable thioether linkage. The coupling was optimized to give a high binding capacity of 5 mg or more of Glutathione-S-transferase (GST) per ml of wet gel. Bound GST–fusion proteins are easily displaced from the resin by elution with buffers containing reduced glutathione. Mild elution conditions preserve protein antigenicity and function.
• Matrix: Agarose microbeads, 4%
• Matrix Activation: Epoxy
• Particle Size Range: 25-50 μm
• Spacer Arm: 12 atoms
• Binding Capacity: 5-10 mg recombinant GST-tagged protein / mL drained gel
• Ligand: Reduced glutathione (GSH)
• Molecular Weight Range: 6 x 104 - 2 x 107
Research or further manufacturing use only, not for food or drug use.