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Bluo-GAL, 100 MG

Price$ 47.52
  • SKU: X-1016-100MG
  • Pack Size: 100 MG
Quantity
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97753-82-7

374.2 Da

C14H16BrNO6

Clear and colorless solution (Sol. 2%, DMF)

-20°C


Description

Bluo-Gal (5-Bromo-3-indolyl β-D-galactopyranoside) is a chromogenic substrate for β-galactosidase (LacZ), used to determine the presence or absence of a cloned DNA inserted in bacteria growing on agar plates.

When it is hydrolyzed by β-galactosidase, the resulting product will form a blue precipitate. The precipitate is insoluble in alcohol and xylenes, thus it is suitable for immunoblotting and immunocytochemical assays.

Upon microscopic examination under polarized light, the precipitated crystals emit a strong signal consisting of yellow reflected light. Because of this property, Bluo-Gal has more potent sensitivity of the staining method for β-galactosidase and has optimal morphological resolution.

Bluo-Gal can be used as an alternative of X-Gal. Lac+ colonies grown in the presence of Bluo-Gal turn a deeper blue color more intense than X-Gal, allowing for easy differentiation between lac+ and lac- colonies.

 

Applications

• Confirmation of cloned DNA insert in bacteria

• Lac gene detection systems

• Detection of β-gal activity by electron microscopy

• Immunoblotting

• Immunocytochemical assays

• Used in conjunction with IPTG for blue-white screening

 

References

• “Improved in situ β-galactosidase staining for histological analysis of transgenic mice”

• “lacZ Transgenic mice to monitor gene expression in embryo and adult”

 

Not for human therapeutic use or for medicinal purposes. For research applications only.

 

0.1 lbs



Research or further manufacturing use only, not for food or drug use.

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