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RNase A, 500 MG

Price$ 235.25
You Save$ 97.25
  • SKU: R-2000-500MG
  • Pack Size: 500 MG
Quantity
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Total price: $ 850.00

Ribonuclease A; Ribonuclease I; Pancreatic Ribonuclease; Ribonucleate 3′-pyrimidinooligonucleotidohydrolase; 2-(3- Aminopropanoylamino)-3-(1H-imidazol -5-yl)propanoic acid

9001-99-4

C9H14N4O3

-20°C


RNase stands for ribonuclease, an RNA digesting enzyme. RNase consists of 124 amino acids in one polypeptide chain with 4 disulphide linkages. Ribonucleases can be divided into endoribonucleases and exoribonucleases. RNases have important roles in RNA degradation and turnover in all organisms. Ribonuclease enzyme can unwind the RNA helix by complexing with single-stranded RNA.
 

RNase A is an endoribonuclease with functions in RNA metabolism and regulation of gene expression. It was found to play roles in diseases such as autoimmune diseases, renal insufficiencies and pancreas disorder. More recently, an antitumor activity was also reported for an RNase A with cytotoxic and cytostatic properties.
 

The members of RNase A superfamily are widely expressed and present in serum and tissues of mammals. The ribonucleolytic activity in the pancreas of ruminants is particularly high, perhaps to digest the large amount of RNA produced by stomach microorganisms. 


Features & Benefits of AG Scientific's RNase A:

  • Chromatographically purified, salt-free, lyophilized powder
  • DNase and protease free
  • Cleaves at the 3'-side of the pyrimidine (uracil or cytosine) phosphate bonds
  • Degrades RNA to cyclic nucleotide monophosphates to 5'-OH and 2'-,3'-cyclic monophosphate

Applications:

RNase A is used to hydrolyze RNA from protein samples. It is compatible for use in RNase protection assays, to remove unspecifically bound RNA, in the analysis of RNA sequences, to hydrolyze RNA contained in protein samples, and in the purification of DNA.


Additional Reading from AG Scientific:


 

0.1 lbs



Research or further manufacturing use only, not for food or drug use.
  • Purity (by SDS Page): Report %
  • Source: Bovine pancreas
  • Form: A white chromatographically purified, salt free, freeze dried powder.
  • Unit Definition: That amount of enzyme causing an absorbance decrease per minute equivalent to the difference between initial and final absorbance of substrate.
  • Activity: Report U/mg
  • Additional Data: Salt free, protease free, D’nase free by spectrophotometric method
  • Method of Assay: The method of assay is based on that of Kunitz, M. (1946) J. Biol. Chem. 164.563 in which the hydrolysis of yeast ribonucleic acid is followed spectrophotometrically by measuring the decrease in absorbance at 300 nm.
  • Storage & Handling: Store in tightly sealed vial at -20°C. Once opened, re-desiccate over silica gel under vacuum before returning to -20˚C.

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