133343-34-7
376.4 Da
C15H24N2O7S
Soluble in DMSO, water or ethanol
-20°C
Description
Lactacystin is a microbial metabolite of Streptomyces strains and acts as a cell permeable, specific, and irreversible inhibitor of 20S proteasome and lysosomal enzyme, Cathepsin A. It irreversibly binds to N-terminal threonine residue on active site of catalytic B subunit of 20S proteasome and therefore blocks proteolytic activity.
The metabolite blocks the cell cycle and induces neurite outgrowth in a murine neuroblastoma cell line, Neuro-2a. It inhibits cell cycle progression in both the G0/G1 and G2/M phases of the cell cycle. Treatment of Lactacystin in Neuro-2a cells displays bipolar morphology and becomes progressively more multipolar.
It has been reported that lactacystin induces apoptosis in human monoblastic U937, confirming DNA fragmentation.
Applications
• Proteasome inhibitor
• Study of neuritogenesis
References
• “Lactacystin, a novel microbial metabolite, induces neuritogenesis of neuroblastoma cells”
• “Lactacystin, Proteasome Function, and Cell Fate”
Not for human therapeutic use or for medicinal purposes. For research applications only.
0.1 lbs
Research or further manufacturing use only, not for food or drug use.
