Proteinase K

SKU
P-1265
  • CAS:39450-01-6
  • MW:28,500 Da
  • Appearance:White to off-white lyophilized solid
  • Purity:≥99%

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Product Name Qty
Proteinase K
500 mg
$199.99
Proteinase K
1 g
$249.99
Proteinase K
50 mg
$39.99
Proteinase K
100 mg
$59.99

Description

Proteinase K (also known as endopeptidase K) is an extracellular endopeptidase isolated from a culture filtrate of the fungus Tritirachium album limber. This unique fungus can degrade keratin (hence Proteinase “K”) and can grow using the by-product as its sole source of carbon and nitrogen.

The enzyme is stable over a broad temperature and pH range, with optimum activity at 55-65°C and pH 8.0. It’s also stable in high salt concentrations and active in the presence of strong detergents such as SDS. It has a molecular weight of 28.5 kDa and it shows fairly-low specificity for its polypeptide targets.

 

Features and Benefits of AG’s Proteinase K

• DNAse and RNAse free via chromatography purification
• 6x greater activity than Pronase and 3x greater activity than bovine trypsin
• Broad substrate specificity
• Versatile in blood, cultured cells, tissues, bacteria, yeast, etc.
• Active and stable over a wide range of conditions
• Compatible with most DNA and RNA isolation buffers

 

Applications in Next Generation Sequencing (NGS) and Microarray Technologies

• Nucleic acid purification by inactivating nucleases when extracting DNA and RNA from yeast, bacteria, and mammalian cell & plant cell lysates
• Improves cloning efficiency of PCR products
• Sample preparation for quantification of DNA adduct levels by accelerator mass spectrometry
• Inactivation of enzyme cocktails in ribonuclease protection assays
• Added to extraction procedures to optimize RNA yields from primary breast tumors for microarray studies

 

Applications with Proteins

• Detection of Bovine Spongiform Encephalopathy proteins which are uniquely resistant to proteolytic degradation.
• Tissue digestion as an alternative sample preparation approach for quantitative analysis using liquid chromatography—tandem mass spectrometry
• Specifically modify cell surface proteins to analyze membrane structures for protein localization
• Generates protein fragments used in characterization of functional studies

 

Specifications

• EC Number: 3.4.21.64 

 

More Information
Alternate Name/Synonyms
Endopeptidase K; Protease K
SKU
P-1265
CAS #
39450-01-6
Molecular Weight
28,500 Da
Appearance
White to off-white lyophilized solid
Purity
≥99%
Solubility
50mM Tris-HCl, 2mM calcium acetate, pH 8.0
Storage Temp
4°C
THERAPEUTIC AREA
Not Available
USE
Proteinase K is extremely effective on native proteins and can therefore be used to rapidly inactivate endogenous nucleases such as RNases and DNases. This property makes Proteinase K particularly suitable for the isolation of native RNA and DNA from tissues or cell lines. It promotes cell lysis by activating a bacterial autolytic factor and is also used for the analysis of membrane structures by modifying proteins and glycoproteins on cell surfaces. It is particularly well suited for isolating nucleic acids for amplification reactions and can be used to remove cellular debris during the preparation of colony lifts, and to treat tissue sections to ensure efficient probe infiltration during in situ hybridization.
MDL NUMBER
MFCD00132129
CHEMACX
X1010077-5
Handling
Use only in area provided with appropriate exhaust ventilation. Keep away from heat and source of ignition. Empty containers pose a fire risk, evaporate residue under fume hood. Ground all equipment containing material. Do not breathe dust.
Additional Videos
msds 1
/media/upload/pdf/P-1265, Proteinase K, SDS, diamond format.pdf
Certificate of Analysis 1
/media/upload/pdf/P-1265, J1298.pdf
Certificate of Analysis 2
/media/upload/pdf/P-1265, J1072I.pdf
Certificate of Analysis 3
media/upload/pdf/P-1265, J1072H.pdf
GHS PICTOGRAMS
Handling Use only in area provided with appropriate exhaust ventilation. Keep away from heat and source of ignition. Empty containers pose a fire risk, evaporate residue under fume hood. Ground all equipment containing material. Do not breathe dust.
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