Puromycin Dihydrochloride

Product Name	Qty
Puromycin, dihydrochloride 500 mg $384.15
Puromycin, dihydrochloride 1 g $471.26
Puromycin, dihydrochloride 25 mg $43.29
Puromycin, dihydrochloride 100 mg $90.20

Description

Puromycin Dihydrochloride is an aminoglycoside antibiotic that is isolated from Streptomyces alboniger. Puromycin acts as a protein synthesis inhibitor by incorporating itself into a polypetide chain that has been growing, causing it to prematurely terminate. Puromycin’s primary application is as a gene-selection antibiotic with an important role in gene-editing via CRISPR/Cas9.

This puromycin reagent, which is in the form of hydrochloride salt, has antineoplastic, antitrypanosomal, and antimicrobial properties. It is important to keep Puromycin Dihydrochloride stored in a tightly closed container in a well-ventilated and dry area.

Applications

• Selective antibiotic agent for cell culture systems, selection of mammalian cell lines
• Protein synthesis inhibitor
• Studies of regulatory mechanisms which control specific gene functions during cell differentiation
• Can be used for specific types of cancer therapy

Specifications

More Information

Alternate Name/Synonyms

Stylomycin hydrochloride; 3′-[α-Amino-p-methoxyhydrocinnamamido]-3′-deoxy-N,N-dimethyladenosine dihydrochloride

SKU

P-1033

CAS #

58-58-2

Chemical Name

(2S)-2-amino-N-[(2S,3R,4S,5R)-5-[6-(dimethylamino)purin-9-yl]-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl]-3-(4-methoxyphenyl)propanamide;dihydrochloride

Chemical Formula

C₂₂H₂₉N₇O₅ • 2HCl

Molecular Weight

544.43 Da

Appearance

White to off-white powder

Purity

≥99% (HPLC)

Solubility

Soluble in water (50 mg/mL), ethanol (~1 mg/mL), DMSO (~13 mg/mL), DMF (~14 mg/mL), and PBS, pH 72 (~10 mg/mL)

Melting Point

175-177 °C

Preparation

N/A

Storage Temp

-20°C

THERAPEUTIC AREA

Infectious Diseases

USE

Puromycin dihydrochloride has been used in the preparation of puromycin stock solution for puromycin sensitivity assay and to select lentivirus-infected cells containing puromycin resistant selection marker. It is a part of the amino-nucleoside family of antibiotics and is derived from Streptomyces alboniger. It is a broad spectrum antibiotic with antitumor activity, as an inhibitor of protein synthesis and has been used to study transcription regulatory mechanisms that control the sequential and coordinate expression of genes during cell differentiation. The recommended working concentration in eukaryotic cell culture is 1-10 μg/mL.

Merck Index

14: 7943

MDL NUMBER

MFCD00866328

CHEMACX

X1001900-0

INCHI

InChI=1S/C22H29N7O5/c1-28(2)19-17-20(25-10-24-19)29(11-26-17)22-18(31)16(15(9-30)34-22)27-21(32)14(23)8-12-4-6-13(33-3)7-5-12/h4-7,10-11,14-16,18,22,30-31H,8-9,23H2,1-3H3,(H,27,32)/t14-,15+,16+,18+,22+/m0/s1

SMILES

CN(C)C1=NC=NC2=C1N=CN2C3C(C(C(O3)CO)NC(=O)C(CC4=CC=C(C=C4)OC)N)O

RTECS

AU7350000

UN #'S

UN 2811

PACKING GROUP

I

Handling

Use only in area provided with appropriate exhaust ventilation. Keep away from heat and source of ignition. Empty containers pose a fire risk, evaporate residue under fume hood. Ground all equipment containing material. Do not breathe dust.

msds 1

/media/upload/pdf/P-1033, Puromycin dihydrochloride, SDS, diamond format.pdf

Certificate of Analysis 1

/media/upload/pdf/P-1033, J1277.pdf

Certificate of Analysis 2

/media/upload/pdf/P-1033, J1006D.pdf

Certificate of Analysis 3

/media/upload/pdf/P-1033, J1006C.pdf

Reviews

Safety

GHS PICTOGRAMS
Handling	Use only in area provided with appropriate exhaust ventilation. Keep away from heat and source of ignition. Empty containers pose a fire risk, evaporate residue under fume hood. Ground all equipment containing material. Do not breathe dust.
UN #'S	UN 2811
Packing Group	I

Citations

Citations	Transferring whole genomes from bacteria to yeast spheroplasts using entire bacterial cells to reduce DNA shearing
	Co-Inflammatory Roles of TGFβ1 in the Presence of TNFα Drive a Pro-inflammatory Fate in Mesenchymal Stem Cells
	Proteomic And Transcriptomic Analyses Reveal Novel Aspects Of Post-Transcriptional Regulation In Saccharomyces cerevisiae
	Identification and characterization of a drug-sensitive strain enables puromycin-based translational assays in Saccharomyces cerevisiae
	Estudo da expressão da enzima desubiquitinante USP2a e de sua interação com a proteina clatrina em celulas derivadas de carcinomas espinocelulares bucais e de prostata humanos
	Dynamics of single mRNP nucleocytoplasmic transport and export through the nuclear pore in living cells
	High TCL1 expression and intact T-cell receptor signaling define a hyperproliferative subset of T-cell prolymphocytic leukemia
	The in vivo kinetics of RNA polymerase II elongation during co-transcriptional splicing
	Inhibition of gastric cancer invasion and metastasis by PLA2G2A, a novel beta-catenin/TCF target gene
	Physical and functional interaction between hMSH5 and c-Abl
	The Aryl Hydrocarbon Receptor is a Critical Regulator of Tissue Factor Stability and an Antithrombotic Target in Uremia
	The crystal structure of KSHV ORF57 reveals dimeric active sites important for protein stability and function
	The genome of Spironucleus salmonicida highlights a fish pathogen adapted to fluctuating environments
	Noncanonical roles of membranous lysyl-tRNA synthetase in transducing cell-substrate signaling for invasive dissemination of colon cancer spheroids in 3D collagen I gels
	Exosome-derived miRNAs and ovarian carcinoma progression
	Breast cancer: coordinated regulation of CCL2 secretion by intracellular glycosaminoglycans and chemokine motifs
	Inflammatory factors of the tumor microenvironment induce plasticity in nontransformed breast epithelial cells: EMT, invasion, and collapse of normally organized breast textures
	Postentry events are responsible for restriction of productive varicella-zoster virus infection in Chinese hamster ovary cells
	Effects of amino acid substitutions at positions 33 and 37 on UDP-glucuronosyltransferase 1A9 (UGT1A9) activity and substrate selectivity
	Ligand-independent regulation of transforming growth factor beta1 expression and cell cycle progression by the aryl hydrocarbon receptor
	CRISPR/Cas9-mediated gene modification and gene knock out in the human-infective parasite Trichomonas vaginalis
	Quantitative image mean squared displacement (iMSD) analysis of the dynamics of profilin 1 at the membrane of live cells
	The aryl hydrocarbon receptor binds to E2F1 and inhibits E2F1-induced apoptosis
	Vascular endothelial-cadherin regulates cytoskeletal tension, cell spreading, and focal adhesions by stimulating RhoA
	Stabilization of the survival motor neuron protein by ASK1
	Brca1 in immunoglobulin gene conversion and somatic hypermutation
	The KSHV portal protein ORF43 is essential for the production of infectious viral particles
	Conditional, Genetically Encoded, Small Molecule-Regulated Inhibition of NFκB Signaling in RPE Cells
	The inflammatory cytokine TNFα cooperates with Ras in elevating metastasis and turns WT-Ras to a tumor-promoting entity in MCF-7 cells
	Carboxylic acid drug-induced DNA nicking in HEK293 cells expressing human UDP-glucuronosyltransferases: role of acyl glucuronide metabolites and glycation pathways
	Loss of REDD1 augments the rate of the overload-induced increase in muscle mass
	AG490 and PF431396 Sensitive Tyrosine Kinase Control the Population Heterogeneity of Basal STAT1 Activity in Ube1l Deficient Cells
	Roles of end-binding 1 protein and gamma-tubulin small complex in cytokinesis and flagella formation of Giardia lamblia
	Two variants of MutS homolog hMSH5: prevalence in humans and effects on protein interaction
	Breast cancer stem cells characterized by CD70 expression preferentially metastasize to the lungs
	Identification of a Novel Microtubule-Binding Protein in Giardia lamblia
	The T-LAK Cell-originated Protein Kinase Signal Pathway Promotes Colorectal Cancer Metastasis
	Castration alters protein balance after high-frequency muscle contraction
	Defining the Roles of p300/CBP (CREB Binding Protein) and S5a in p53 Polyubiquitination, Degradation and DNA Damage Responses: A Dissertation
	A Trichomonas vaginalis Rhomboid Protease and Its Substrate Modulate Parasite Attachment and Cytolysis of Host Cells
	REDD2 expression in rat skeletal muscle correlates with nutrient-induced activation of mTORC1: responses to aging, immobilization, and remobilization
	The mTORC1 signaling repressors REDD1/2 are rapidly induced and activation of p70S6K1 by leucine is defective in skeletal muscle of an immobilized rat hindlimb
	Repressors of mTORC1 act to blunt the anabolic response to feeding in the soleus muscle of a cast-immobilized mouse hindlimb
	Characterization of Trichomonas vaginalis rhomboid proteases and surface proteins that contribute to host: parasite interactions
	Gentherapeutische Modelle zur anti-angiogenen Therapie experimenteller Tumoren
	A Proteomic approach for protein-profiling the oncogenic ras induced transformation (H-, K-, and N-Ras) in NIH/3T3 mouse embryonic fibroblasts
	Role of gamma-giardin in ventral disc formation of Giardia lamblia

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