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Puromycin Dihydrochloride

SKU
P-1033
  • CAS:58-58-2
  • Formula:C22H29N7O5 • 2HCl
  • MW:544.43 Da
  • Appearance:White to off-white powder
  • Purity:≥99% (HPLC)

Starting at $44.87

Product Name Qty
Puromycin, dihydrochloride
25 mg
$44.87
Puromycin, dihydrochloride
100 mg
$92.87
Puromycin, dihydrochloride
500 mg
$401.73
Puromycin, dihydrochloride
1 g
$547.82
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Description

Puromycin Dihydrochloride is an aminoglycoside antibiotic that is isolated from Streptomyces alboniger. Puromycin acts as a protein synthesis inhibitor by incorporating itself into a polypetide chain that has been growing, causing it to prematurely terminate. Puromycin’s primary application is as a gene-selection antibiotic with an important role in gene-editing via CRISPR/Cas9.

This puromycin reagent, which is in the form of hydrochloride salt, has antineoplastic, antitrypanosomal, and antimicrobial properties. It is important to keep Puromycin Dihydrochloride stored in a tightly closed container in a well-ventilated and dry area.

 

Applications

• Selective antibiotic agent for cell culture systems, selection of mammalian cell lines
• Protein synthesis inhibitor
• Studies of regulatory mechanisms which control specific gene functions during cell differentiation
• Can be used for specific types of cancer therapy

 

Not for human therapeutic use or for medicinal purposes. For research applications only.

More Information
Alternate Name/Synonyms
Stylomycin hydrochloride; 3′-[α-Amino-p-methoxyhydrocinnamamido]-3′-deoxy-N,N-dimethyladenosine dihydrochloride
SKU
P-1033
CAS #
58-58-2
Chemical Name
(2S)-2-amino-N-[(2S,3R,4S,5R)-5-[6-(dimethylamino)purin-9-yl]-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl]-3-(4-methoxyphenyl)propanamide;dihydrochloride
Chemical Formula
C22H29N7O5 • 2HCl
Molecular Weight
544.43 Da
Appearance
White to off-white powder
Purity
≥99% (HPLC)
Solubility
Soluble in water (50 mg/mL), ethanol (~1 mg/mL), DMSO (~13 mg/mL), DMF (~14 mg/mL), and PBS, pH 72 (~10 mg/mL)
Melting Point
175-177 °C
Storage Temp
-20°C
THERAPEUTIC AREA
Infectious Diseases
USE
Puromycin dihydrochloride has been used in the preparation of puromycin stock solution for puromycin sensitivity assay and to select lentivirus-infected cells containing puromycin resistant selection marker. It is a part of the amino-nucleoside family of antibiotics and is derived from Streptomyces alboniger. It is a broad spectrum antibiotic with antitumor activity, as an inhibitor of protein synthesis and has been used to study transcription regulatory mechanisms that control the sequential and coordinate expression of genes during cell differentiation. The recommended working concentration in eukaryotic cell culture is 1-10 μg/mL.
Merck Index
14: 7943
MDL NUMBER
MFCD00866328
CHEMACX
X1001900-0
INCHI
InChI=1S/C22H29N7O5/c1-28(2)19-17-20(25-10-24-19)29(11-26-17)22-18(31)16(15(9-30)34-22)27-21(32)14(23)8-12-4-6-13(33-3)7-5-12/h4-7,10-11,14-16,18,22,30-31H,8-9,23H2,1-3H3,(H,27,32)/t14-,15+,16+,18+,22+/m0/s1
SMILES
CN(C)C1=NC=NC2=C1N=CN2C3C(C(C(O3)CO)NC(=O)C(CC4=CC=C(C=C4)OC)N)O
RTECS
AU7350000
UN #'S
UN 2811
PACKING GROUP
I
Handling
Use only in area provided with appropriate exhaust ventilation. Keep away from heat and source of ignition. Empty containers pose a fire risk, evaporate residue under fume hood. Ground all equipment containing material. Do not breathe dust.
msds 1
P-1033, Puromycin dihydrochloride, SDS, diamond format.pdf
Certificate of Analysis 1
P-1033, L1435.pdf
Certificate of Analysis 2
P-1033, J1332 r01.pdf
Certificate of Analysis 3
P-1033, K1412B.pdf
GHS PICTOGRAMS
HandlingUse only in area provided with appropriate exhaust ventilation. Keep away from heat and source of ignition. Empty containers pose a fire risk, evaporate residue under fume hood. Ground all equipment containing material. Do not breathe dust.
UN #'SUN 2811
Packing GroupI
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Co-Inflammatory Roles of TGFβ1 in the Presence of TNFα Drive a Pro-inflammatory Fate in Mesenchymal Stem Cells
Proteomic And Transcriptomic Analyses Reveal Novel Aspects Of Post-Transcriptional Regulation In Saccharomyces cerevisiae
Identification and characterization of a drug-sensitive strain enables puromycin-based translational assays in Saccharomyces cerevisiae
Estudo da expressão da enzima desubiquitinante USP2a e de sua interação com a proteina clatrina em celulas derivadas de carcinomas espinocelulares bucais e de prostata humanos
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The in vivo kinetics of RNA polymerase II elongation during co-transcriptional splicing
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Physical and functional interaction between hMSH5 and c-Abl
The Aryl Hydrocarbon Receptor is a Critical Regulator of Tissue Factor Stability and an Antithrombotic Target in Uremia
The crystal structure of KSHV ORF57 reveals dimeric active sites important for protein stability and function
The genome of Spironucleus salmonicida highlights a fish pathogen adapted to fluctuating environments
Noncanonical roles of membranous lysyl-tRNA synthetase in transducing cell-substrate signaling for invasive dissemination of colon cancer spheroids in 3D collagen I gels
Exosome-derived miRNAs and ovarian carcinoma progression
Breast cancer: coordinated regulation of CCL2 secretion by intracellular glycosaminoglycans and chemokine motifs
Inflammatory factors of the tumor microenvironment induce plasticity in nontransformed breast epithelial cells: EMT, invasion, and collapse of normally organized breast textures
Postentry events are responsible for restriction of productive varicella-zoster virus infection in Chinese hamster ovary cells
Effects of amino acid substitutions at positions 33 and 37 on UDP-glucuronosyltransferase 1A9 (UGT1A9) activity and substrate selectivity
Ligand-independent regulation of transforming growth factor beta1 expression and cell cycle progression by the aryl hydrocarbon receptor
CRISPR/Cas9-mediated gene modification and gene knock out in the human-infective parasite Trichomonas vaginalis
Quantitative image mean squared displacement (iMSD) analysis of the dynamics of profilin 1 at the membrane of live cells
The aryl hydrocarbon receptor binds to E2F1 and inhibits E2F1-induced apoptosis
Vascular endothelial-cadherin regulates cytoskeletal tension, cell spreading, and focal adhesions by stimulating RhoA
Stabilization of the survival motor neuron protein by ASK1
Brca1 in immunoglobulin gene conversion and somatic hypermutation
The KSHV portal protein ORF43 is essential for the production of infectious viral particles
Conditional, Genetically Encoded, Small Molecule-Regulated Inhibition of NFκB Signaling in RPE Cells
The inflammatory cytokine TNFα cooperates with Ras in elevating metastasis and turns WT-Ras to a tumor-promoting entity in MCF-7 cells
Carboxylic acid drug-induced DNA nicking in HEK293 cells expressing human UDP-glucuronosyltransferases: role of acyl glucuronide metabolites and glycation pathways
Loss of REDD1 augments the rate of the overload-induced increase in muscle mass
AG490 and PF431396 Sensitive Tyrosine Kinase Control the Population Heterogeneity of Basal STAT1 Activity in Ube1l Deficient Cells
Roles of end-binding 1 protein and gamma-tubulin small complex in cytokinesis and flagella formation of Giardia lamblia
Two variants of MutS homolog hMSH5: prevalence in humans and effects on protein interaction
Breast cancer stem cells characterized by CD70 expression preferentially metastasize to the lungs
Identification of a Novel Microtubule-Binding Protein in Giardia lamblia
The T-LAK Cell-originated Protein Kinase Signal Pathway Promotes Colorectal Cancer Metastasis
Castration alters protein balance after high-frequency muscle contraction
Defining the Roles of p300/CBP (CREB Binding Protein) and S5a in p53 Polyubiquitination, Degradation and DNA Damage Responses: A Dissertation
A Trichomonas vaginalis Rhomboid Protease and Its Substrate Modulate Parasite Attachment and Cytolysis of Host Cells
REDD2 expression in rat skeletal muscle correlates with nutrient-induced activation of mTORC1: responses to aging, immobilization, and remobilization
The mTORC1 signaling repressors REDD1/2 are rapidly induced and activation of p70S6K1 by leucine is defective in skeletal muscle of an immobilized rat hindlimb
Repressors of mTORC1 act to blunt the anabolic response to feeding in the soleus muscle of a cast-immobilized mouse hindlimb
Characterization of Trichomonas vaginalis rhomboid proteases and surface proteins that contribute to host: parasite interactions
Gentherapeutische Modelle zur anti-angiogenen Therapie experimenteller Tumoren
A Proteomic approach for protein-profiling the oncogenic ras induced transformation (H-, K-, and N-Ras) in NIH/3T3 mouse embryonic fibroblasts
Role of gamma-giardin in ventral disc formation of Giardia lamblia
Neuromedin U (NMU) regulates osteoblast differentiation and activity
Contributions of the four essential entry glycoproteins to HSV-1 tropism and the selection of entry routes.
A novel voluntary weightlifting model in mice promotes muscle adaptation and insulin sensitivity with simultaneous enhancement of autophagy and mTOR pathway
A Time Course for Markers of Protein Synthesis and Degradation with Hindlimb Unloading and the Accompanying Anabolic Resistance to Refeeding
Lytic Reactivation of the Kaposi\'s sarcoma-associated herpesvirus (KSHV) is Accompanied by Major Nucleolar Alterations
The Sub-Nuclear Localization of RNA-Binding Proteins in KSHV-Infected Cells
Targeting the Kaposi’s Sarcoma-associated Herpesvirus Genome With the CRISPR-Cas9 Platform in Latently Infected Cells
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