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Puromycin Dihydrochloride

SKU
P-1033
  • CAS:58-58-2
  • Formula:C22H29N7O5 • 2HCl
  • MW:544.43 Da
  • Appearance:White to off-white powder
  • Purity:≥99% (HPLC)
Product Name Qty
Puromycin, dihydrochloride
25 mg
$44.87
Puromycin, dihydrochloride
100 mg
$92.87
Puromycin, dihydrochloride
500 mg
$401.73
Puromycin, dihydrochloride
1 g
$547.82
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Description

Puromycin Dihydrochloride is an aminoglycoside antibiotic that is isolated from Streptomyces alboniger. Puromycin acts as a protein synthesis inhibitor by incorporating itself into a polypetide chain that has been growing, causing it to prematurely terminate. Puromycin’s primary application is as a gene-selection antibiotic with an important role in gene-editing via CRISPR/Cas9.

This puromycin reagent, which is in the form of hydrochloride salt, has antineoplastic, antitrypanosomal, and antimicrobial properties. It is important to keep Puromycin Dihydrochloride stored in a tightly closed container in a well-ventilated and dry area.

 

Applications

• Selective antibiotic agent for cell culture systems, selection of mammalian cell lines
• Protein synthesis inhibitor
• Studies of regulatory mechanisms which control specific gene functions during cell differentiation
• Can be used for specific types of cancer therapy

 

Not for human therapeutic use or for medicinal purposes. For research applications only.

More Information
Alternate Name/Synonyms
Stylomycin hydrochloride; 3′-[α-Amino-p-methoxyhydrocinnamamido]-3′-deoxy-N,N-dimethyladenosine dihydrochloride
SKU
P-1033
CAS #
58-58-2
Chemical Name
(2S)-2-amino-N-[(2S,3R,4S,5R)-5-[6-(dimethylamino)purin-9-yl]-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl]-3-(4-methoxyphenyl)propanamide;dihydrochloride
Chemical Formula
C22H29N7O5 • 2HCl
Molecular Weight
544.43 Da
Appearance
White to off-white powder
Purity
≥99% (HPLC)
Solubility
Soluble in water (50 mg/mL), ethanol (~1 mg/mL), DMSO (~13 mg/mL), DMF (~14 mg/mL), and PBS, pH 72 (~10 mg/mL)
Melting Point
175-177 °C
Storage Temp
-20°C
THERAPEUTIC AREA
Infectious Diseases
USE
Puromycin dihydrochloride has been used in the preparation of puromycin stock solution for puromycin sensitivity assay and to select lentivirus-infected cells containing puromycin resistant selection marker. It is a part of the amino-nucleoside family of antibiotics and is derived from Streptomyces alboniger. It is a broad spectrum antibiotic with antitumor activity, as an inhibitor of protein synthesis and has been used to study transcription regulatory mechanisms that control the sequential and coordinate expression of genes during cell differentiation. The recommended working concentration in eukaryotic cell culture is 1-10 μg/mL.
Merck Index
14: 7943
MDL NUMBER
MFCD00866328
CHEMACX
X1001900-0
INCHI
InChI=1S/C22H29N7O5/c1-28(2)19-17-20(25-10-24-19)29(11-26-17)22-18(31)16(15(9-30)34-22)27-21(32)14(23)8-12-4-6-13(33-3)7-5-12/h4-7,10-11,14-16,18,22,30-31H,8-9,23H2,1-3H3,(H,27,32)/t14-,15+,16+,18+,22+/m0/s1
SMILES
CN(C)C1=NC=NC2=C1N=CN2C3C(C(C(O3)CO)NC(=O)C(CC4=CC=C(C=C4)OC)N)O
RTECS
AU7350000
UN #'S
UN 2811
PACKING GROUP
I
Handling
Use only in area provided with appropriate exhaust ventilation. Keep away from heat and source of ignition. Empty containers pose a fire risk, evaporate residue under fume hood. Ground all equipment containing material. Do not breathe dust.
msds 1
P-1033, Puromycin dihydrochloride, SDS, diamond format.pdf
Certificate of Analysis 1
P-1033, L1435.pdf
Certificate of Analysis 2
P-1033, J1332 r01.pdf
Certificate of Analysis 3
P-1033, K1412B.pdf
GHS PICTOGRAMS
HandlingUse only in area provided with appropriate exhaust ventilation. Keep away from heat and source of ignition. Empty containers pose a fire risk, evaporate residue under fume hood. Ground all equipment containing material. Do not breathe dust.
UN #'SUN 2811
Packing GroupI
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A Time Course for Markers of Protein Synthesis and Degradation with Hindlimb Unloading and the Accompanying Anabolic Resistance to Refeeding
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