Puromycin Dihydrochloride Solution

SKU
P-1033-SOL
  • CAS:58-58-2
  • Formula:C22H29N7O5 • 2HCl
  • MW:544.43 Da
  • Appearance:Clear and colorless to light yellow solution
  • Purity:≥99% (HPLC)

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Starting at $117.25

Product Name Qty
Puromycin dihydrochloride Solution
5x2 ml
$117.25

DESCRIPTION

AG Scientific's Puromycin dihydrochloride solution is a sterile filtrate solution.

Puromycin is an aminonucleoside antibiotic that causes early chain termination within the ribosome during translation. This inhibition of translation is effective in both in vivo and in vitro applications, making it an ideal tool for protein synthesis studies. While selection for E. Coli cells is possible with puromycin, it is more commonly and effectively used for mammalian cell selection.

It is active against gram-positive microorganisms, less active against acid-fast bacilli and more-weakly active against gram-negative microorganisms. The growth of bacteria, protozoa, algae and mammalian cells is quickly halted by puromycin. However, cells with the pac gene have resistance, its gene product is puromycin-N-acetyl-transferase which inactivates puromycin through acetylation. Research has shown puromycin to be effective against certain tumors.

Recently researchers have developed a prodrug strategy for selective cancer therapy using a masked cytotoxic agent Fmoc-Lys(Ac)-Puromycin, which can be sequentially activated by histone deacetylases (HDACs) and cathepsin L (CTSL) to kill cancer cells expressing high levels of both enzymes. The data gathered indicates that Fmoc-Lys(Ac)-Puromycin can substantially improve anti-cancer efficacy and can be developed into potential selective cancer therapy.

 

APPLICATIONS

• Puromycin is soluble in water and is often used as a selective agent in molecular biology research.
• Puromycin has a longstanding application as a gene selection antibiotic and a powerful new role in Genome editing utilizing the CRISPR/Cas9 system.

 

SPECIFICATIONS

Purity data is for a concentration of 10 mg/ml.

 

More Information
Alternate Name/Synonyms
Stylomycin hydrochloride; 3′-[α-Amino-p-methoxyhydrocinnamamido]-3′-deoxy-N,N-dimethyladenosine dihydrochloride
SKU
P-1033-SOL
CAS #
58-58-2
Chemical Name
(2S)-2-amino-N-[(2S,3R,4S,5R)-5-[6-(dimethylamino)purin-9-yl]-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl]-3-(4-methoxyphenyl)propanamide;dihydrochloride
Chemical Formula
C22H29N7O5 • 2HCl
Molecular Weight
544.43 Da
Appearance
Clear and colorless to light yellow solution
Purity
≥99% (HPLC)
Solubility
Soluble in water (50 mg/mL), ethanol (~1 mg/mL), DMSO (~13 mg/mL), DMF (~14 mg/mL), and PBS, pH 72 (~10 mg/mL)
Melting Point
N/A
Preparation
N/A
Storage Temp
-20°C
THERAPEUTIC AREA
Infectious Diseases
USE
Puromycin dihydrochloride has been used in the preparation of puromycin stock solution for puromycin sensitivity assay and to select lentivirus-infected cells containing puromycin resistant selection marker. It is a part of the amino-nucleoside family of antibiotics and is derived from Streptomyces alboniger. It is a broad spectrum antibiotic with antitumor activity, as an inhibitor of protein synthesis and has been used to study transcription regulatory mechanisms that control the sequential and coordinate expression of genes during cell differentiation. The recommended working concentration in eukaryotic cell culture is 1-10 μg/mL.
Merck Index
13.8044.2001
MDL NUMBER
MFCD00012691
CHEMACX
X1017033-1
INCHI
InChI=1S/C22H29N7O5.2ClH/c1-28(2)19-17-20(25-10-24-19)29(11-26-17)22-18(31)16(15(9-30)34-22)27-21(32)14(23)8-12-4-6-13(33-3)7-5-12;;/h4-7,10-11,14-16,18,22,30-31H,8-9,23H2,1-3H3,(H,27,32);2*1H/t14-,15+,16+,18+,22+;;/m0../s1
SMILES
CN(C)c1c2c(ncn1)n(cn2)[C@H]3[C@@H]([C@@H]([C@H](O3)CO)NC(=O)[C@H](Cc4ccc(cc4)OC)N)O.Cl.Cl
RTECS
N/A
Handling
Use only in area provided with appropriate exhaust ventilation. Keep away from heat and source of ignition. Empty containers pose a fire risk, evaporate residue under fume hood. Ground all equipment containing material. Do not breathe dust.
Certificate of Analysis 1
Certificate of Analysis 2
Certificate of Analysis 3
GHS PICTOGRAMS
Handling Use only in area provided with appropriate exhaust ventilation. Keep away from heat and source of ignition. Empty containers pose a fire risk, evaporate residue under fume hood. Ground all equipment containing material. Do not breathe dust.
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