Pyruvate Oxidase, Pox
- CAS:9001-96-1
- MW:~260 kDa
- Appearance:Lyophilized, yellowish amorphous powder
SDS
CofA
Description
Pyruvate oxidase (E.C. 1.2.3.3) is originated from Aerococcus viridans and used as a component of screen-printed phosphate biosensor for monitoring phosphate concentrations in a sequencing batch reactor (SBR) system. It consumes phosphate in the presence of pyruvate and oxygen and generates hydrogen peroxide, carbon dioxide and acetylphosphate.
The enzyme is a flavoprotein with the relatively tightly bound cofactors flavin adenine dinucleotide, thiamine pyrophosphate, and a divalent metal ion, with Mn2+ being the most effective.
Activity: 40 units/mg powder. One unit is defined as amount of enzyme required to generate 1umol of H202 per minute at 37C, pH 6.5. Contaminate info: Glutamate oxalacetate transaminase (GOT) 002% by activity; Glutamate pyruvate transaminase (GPT) 006% by activity; Lactate Oxidase 002% by activity; Catalase not detectable.
Applications
• Study of aerobic metabolism of bacteria
• Used for enzymatic determination of AST and ALT
• Used to monitor phosphate concentration in SBR system
References
• “Purification and biochemical characterization of pyruvate oxidase from Lactobacillus plantarum”
• “Role of the divalent metal cation in the pyruvate oxidase reaction.”
Not for human therapeutic use or for medicinal purposes. For research applications only.
| Handling | Use only in area provided with appropriate exhaust ventilation. Keep away from heat and source of ignition. Empty containers pose a fire risk, evaporate residue under fume hood. Ground all equipment containing material. Do not breathe dust. |
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