Pyruvate Oxidase, Pox
SKU
P-1600
- CAS:9001-96-1
- MW:~260 kDa
- Appearance:Lyophilized Powder
SDS
CofAStarting at $201.01
Pyruvate oxidase is a peripheral membrane enzyme isolated from Escherichia coli. Pyruvate oxidase catalyzes the oxidative carboxylation of pyruvate to yield the phosphoryl donor, acetyl phosphate releasing CO2 and H2O2. Most bacterial pyruvate oxidases require Mg(II) to bind the thiamine pyrophosphate cofactor in the active site.
Activity: 40 units/mg powder. One unit is defined as amount of enzyme required to generate 1umol of H202 per minute at 37C, pH 6.5. Contaminate info: Glutamate oxalacetate transaminase (GOT) 002% by activity; Glutamate pyruvate transaminase (GPT) 006% by activity; Lactate Oxidase 002% by activity; Catalase not detectable.
More Information
Alternate Name/Synonyms
Pyruvate:oxygen oxidoreductase (phosphorylating)
SKU
P-1600
CAS #
9001-96-1
Chemical Name
2-OXOPROPANOICACID OXIDASE
Chemical Formula
N/A
Molecular Weight
~260 kDa
Appearance
Lyophilized Powder
Purity
N/A
Solubility
N/A
Melting Point
N/A
Preparation
N/A
Storage Temp
-20°C
THERAPEUTIC AREA
Not Applicable
USE
Pyruvate Oxidase (PoxB) converts pyruvate directly to acetate and carbon dioxide. It is used to study pyruvate metabolism. It is used to study aerobic metabolism of bacterium, such as Lactobacillus plantarumand Streptococcus pneumoniae. Pyruvate Oxidase is used for enzymatic determination of pyruvate, GOT, and GPT in clinical analysis. Pyruvate Oxidase from microorganisms has been used in the Amplex Red-based fluorescence assay for pyruvate. It has also been used in developing near real-time continuous detection system for pyruvate.
Merck Index
N/A
MDL NUMBER
MFCD00132148
CHEMACX
X1094648-4
Handling
Use only in area provided with appropriate exhaust ventilation. Keep away from heat and source of ignition. Empty containers pose a fire risk, evaporate residue under fume hood. Ground all equipment containing material. Do not breathe dust.
Certificate of Analysis 1
Certificate of Analysis 2
Certificate of Analysis 3
| Handling | Use only in area provided with appropriate exhaust ventilation. Keep away from heat and source of ignition. Empty containers pose a fire risk, evaporate residue under fume hood. Ground all equipment containing material. Do not breathe dust. |
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