Pyruvate Oxidase, Pox

SKU
P-1600
  • CAS:9001-96-1
  • MW:~260 kDa
  • Appearance:Lyophilized, yellowish amorphous powder

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Product Name Qty
Pyruvate Oxidase
1 KU
$205.03

Description

Pyruvate oxidase (E.C. 1.2.3.3) is originated from Aerococcus viridans and used as a component of screen-printed phosphate biosensor for monitoring phosphate concentrations in a sequencing batch reactor (SBR) system. It consumes phosphate in the presence of pyruvate and oxygen and generates hydrogen peroxide, carbon dioxide and acetylphosphate.

The enzyme is a flavoprotein with the relatively tightly bound cofactors flavin adenine dinucleotide, thiamine pyrophosphate, and a divalent metal ion, with Mn2+ being the most effective.

Activity: 40 units/mg powder. One unit is defined as amount of enzyme required to generate 1umol of H202 per minute at 37C, pH 6.5. Contaminate info: Glutamate oxalacetate transaminase (GOT) 002% by activity; Glutamate pyruvate transaminase (GPT) 006% by activity; Lactate Oxidase 002% by activity; Catalase not detectable.

 

Applications

• Study of aerobic metabolism of bacteria

• Used for enzymatic determination of AST and ALT

• Used to monitor phosphate concentration in SBR system

 

Additional Reading

• Electrochemical Processes for Treating Water and Why it Matters

• Single-Use Biosensors for the Detection of Biomarkers

• Advances in Lab-on-a-Chip Technology

 

References

• “Purification and biochemical characterization of pyruvate oxidase from Lactobacillus plantarum”

• “A screen-printed biosensor using pyruvate oxidase for rapid determination of phosphate in synthetic wastewater”

• “Role of the divalent metal cation in the pyruvate oxidase reaction.”

 

Not for human therapeutic use or for medicinal purposes. For research applications only.

More Information
Alternate Name/Synonyms
Oxidase, Pyruvate; Pyruvic oxidase; phosphate-dependent pyruvate oxidase
SKU
P-1600
CAS #
9001-96-1
Chemical Name
2-OXOPROPANOICACID OXIDASE
Molecular Weight
~260 kDa
Appearance
Lyophilized, yellowish amorphous powder
Storage Temp
-20°C
THERAPEUTIC AREA
Not Applicable
USE
Pyruvate Oxidase (PoxB) converts pyruvate directly to acetate and carbon dioxide. It is used to study pyruvate metabolism. It is used to study aerobic metabolism of bacterium, such as Lactobacillus plantarumand Streptococcus pneumoniae. Pyruvate Oxidase is used for enzymatic determination of pyruvate, GOT, and GPT in clinical analysis. Pyruvate Oxidase from microorganisms has been used in the Amplex Red-based fluorescence assay for pyruvate. It has also been used in developing near real-time continuous detection system for pyruvate.
MDL NUMBER
MFCD00132148
CHEMACX
X1094648-4
Handling
Use only in area provided with appropriate exhaust ventilation. Keep away from heat and source of ignition. Empty containers pose a fire risk, evaporate residue under fume hood. Ground all equipment containing material. Do not breathe dust.
Certificate of Analysis 1
Certificate of Analysis 2
Certificate of Analysis 3
Handling Use only in area provided with appropriate exhaust ventilation. Keep away from heat and source of ignition. Empty containers pose a fire risk, evaporate residue under fume hood. Ground all equipment containing material. Do not breathe dust.
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