Description

Pyruvate oxidase (E.C. 1.2.3.3) is originated from Aerococcus viridans and used as a component of screen-printed phosphate biosensor for monitoring phosphate concentrations in a sequencing batch reactor (SBR) system. It consumes phosphate in the presence of pyruvate and oxygen and generates hydrogen peroxide, carbon dioxide and acetylphosphate.

The enzyme is a flavoprotein with the relatively tightly bound cofactors flavin adenine dinucleotide, thiamine pyrophosphate, and a divalent metal ion, with Mn2+ being the most effective.

Activity: 40 units/mg powder. One unit is defined as amount of enzyme required to generate 1umol of H202 per minute at 37C, pH 6.5. Contaminate info: Glutamate oxalacetate transaminase (GOT) 002% by activity; Glutamate pyruvate transaminase (GPT) 006% by activity; Lactate Oxidase 002% by activity; Catalase not detectable.

Applications

• Study of aerobic metabolism of bacteria

• Used for enzymatic determination of AST and ALT

• Used to monitor phosphate concentration in SBR system

Additional Reading

• Electrochemical Processes for Treating Water and Why it Matters

• Single-Use Biosensors for the Detection of Biomarkers

• Advances in Lab-on-a-Chip Technology

References

• “Purification and biochemical characterization of pyruvate oxidase from Lactobacillus plantarum”

• “A screen-printed biosensor using pyruvate oxidase for rapid determination of phosphate in synthetic wastewater”

• “Role of the divalent metal cation in the pyruvate oxidase reaction.”

Not for human therapeutic use or for medicinal purposes. For research applications only.