TBE (TRIS-Borate-EDTA) Buffer 10X Powder

SKU
T-2406
  • CAS:10043-35-3
  • Formula:H3BO3
  • MW:61.83 Da
  • Appearance:White powder

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Product Name Qty
TBE, buffer, 10X Powder
10 L
$114.99

Description

Tris-Borate-EDTA (TBE) buffer is commonly used in DNA and RNA polyacrylamide gel electrophoresis and is also used for DNA automated sequencing gel. TBE is compatible with both non-denaturing and denaturing gels. It can be used for agarose gels but is not recommended for preparative gels for the recovery of nucleic acids. TBE has been utilized for pulsed-field gel electrophoresis (PFGE) as well.

This product is a 10x concentrate, dry powder, homogeneous blend of molecular biology grade TRIS base, Boric Acid and EDTA.. Ready to use in gel electrophoresis after dilution.

Specifications

•pH (1X solution): 8.20-8.40

More Information
Alternate Name/Synonyms
TBE buffer
SKU
T-2406
CAS #
10043-35-3
Chemical Name
Boric acid
Chemical Formula
H3BO3
Molecular Weight
61.83 Da
Appearance
White powder
Purity
N/A
Solubility
Soluble in water
Melting Point
N/A
Preparation
N/A
Storage Temp
Room temperature
THERAPEUTIC AREA
Not Applicable
USE
TBE (TRIS-Borate-EDTA) Buffer 10X Powder is recommended for gel electrophoresis of RNA and DNA fragments. It is used both as a running buffer and as a gel preparation buffer. It can be filtered through a 0.22 ┬Ám membrane.
Merck Index
N/A
MDL NUMBER
MFCD00236358
CHEMACX
X1096486-2
INCHI
InChI=1S/C10H16N2O8.C4H11NO3.BH3O3/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8;2-1(3)4/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2;2-4H
SMILES
B(O)(O)O.C(CN(CC(=O)O)CC(=O)O)N(CC(=O)O)CC(=O)O.C(C(CO)(CO)N)O
Peptide sequence
Not Applicable
RTECS
Not Applicable
UN #'S
Not Applicable
PACKING GROUP
Not Applicable
HAZARD CLASS
Not Applicable
Handling
Avoid contact with skin, eyes and clothing. Handle in accordance with good industrial hygiene and safety practice.
GHS PICTOGRAMS
Handling Avoid contact with skin, eyes and clothing. Handle in accordance with good industrial hygiene and safety practice.
UN #'S Not Applicable
Packing Group Not Applicable
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