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X-Gal

SKU
X-1015
  • CAS:7240-90-6
  • Formula:C14H15BrClNO6
  • MW:408.61 Da
  • Appearance:White powder
  • Purity:≥ 99% (TLC)
Product Name Qty
X-GAL
1 g
$40.78
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Description

X-Gal, formally referred to as 5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside, is commonly used in molecular biology in regards to cloning applications. First synthesized in 1964, this organic compound contains galactose, which is also found in casein, yogurt, and milk, as well as certain dairy-free products.

An analog of lactose, X-Gal is hydrolyzed by the β-galactosidase enzyme, resulting in 5,5'-dibromo-4,4'-dichloro-indigo -- an insoluble blue precipitate. This indicates enzymatic activity. For example, X-Gal can be used in molecular biology to detect genetic activity in cells and tissues.

 

Applications

Commonly used in blue/white screening, X-Gal yields a visual indication regarding the cellular expression of a functional β-galactosidase enzyme. In turn, key recombinant colonies can be easily identified and then cultured.

 

Not for human therapeutic use or for medicinal purposes. For research applications only.

More Information
Alternate Name/Synonyms
BCIG; Indoxyl-Gal; XGal
SKU
X-1015
CAS #
7240-90-6
Chemical Name
(2S,3R,4S,5R,6R)-2-[(5-bromo-4-chloro-1H-indol-3-yl)oxy]-6-(hydroxymethyl)oxane-3,4,5-triol
Chemical Formula
C14H15BrClNO6
Molecular Weight
408.61 Da
Appearance
White powder
Purity
≥ 99% (TLC)
Solubility
Soluble 2% in DMF
Melting Point
236-238 °C
Storage Temp
-20°C
THERAPEUTIC AREA
Genetic Disorders
USE
X-Gal is a histochemical substrate for β-galactosidase, and it is cleaved by β-galactosidase to yield an insoluble blue precipitate. X-Gal is used to detect DNA insertions into the lacZ region of plasmid DNA, which causes the loss of β-galactosidase activity. It is also used in conjunction with IPTG for detection of b-Galactosidase activity in bacterial colonies in a colorimetric assay.
MDL NUMBER
MFCD00005666
CHEMACX
X1009969-1
INCHI
InChI=1S/C14H15BrClNO6/c15-5-1-2-6-9(10(5)16)7(3-17-6)22-14-13(21)12(20)11(19)8(4-18)23-14/h1-3,8,11-14,17-21H,4H2/t8-,11+,12+,13-,14-/m1/s1
SMILES
c1cc(c(c2c1[nH]cc2O[C@H]3[C@@H]([C@H]([C@H]([C@H](O3)CO)O)O)O)Cl)Br
Handling
Use only in area provided with appropriate exhaust ventilation. Keep away from heat and source of ignition. Empty containers pose a fire risk, evaporate residue under fume hood. Ground all equipment containing material. Do not breathe dust.
msds 1
X-1015, X-Gal, SDS, diamond format.pdf
Certificate of Analysis 1
X-1015, L1511.pdf
Certificate of Analysis 2
X-1015, L1053.pdf
Certificate of Analysis 3
X-1015, H1100A.pdf
GHS PICTOGRAMS
HandlingUse only in area provided with appropriate exhaust ventilation. Keep away from heat and source of ignition. Empty containers pose a fire risk, evaporate residue under fume hood. Ground all equipment containing material. Do not breathe dust.
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