Tips for Selecting Biological Detergents

Biological detergents are chemicals classified as surfactants, or surface-active molecules.

Research scientists typically use these detergents in affecting the interactions within molecules of a biological sample being studied. These surfactants are used in biochemical research to target and manipulate the structure of membrane proteins without sacrificing their integrity or impacting their original function.

Membrane proteins are otherwise classified as lipids and enzymes, among other forms of molecules within a cell membrane.

Even though scientists know a lot more now about the structure of membrane proteins, there are so many types and concentrations of biological detergents, that selecting the wrong type to extract or affect a protein can make or break an entire study.

Types of Biological Detergents

Many of these surfactants are most commonly classified by their ion charge in what is called the head group. Think of head groups as the brain of a molecule — it often contains multiple control centers.

There are ionic, nonionic, and zwitterionic detergents. Based on the type of detergent selected, they can successfully create hydrophobic and hydrophilic interactions in a sample.

Ionic Detergents

The head group of ionic biological detergents either have a positive or negative charge as well as a hydrophobic tail, and are chemically stronger than non-charged detergents. They are a great choice if needed to bind to proteins and alter their structure.

However, it should be noted that due to their strength, ionic detergents more often than not involve some level of protein denaturation.

Non-Ionic Detergents

In contrast to ionic, non-ionic detergents have no charge in their head group, and can be further separated into two groups: polyoxyethylene and glycosidic compounds.

There are differences among the varieties of detergents in either group. However,  the main difference is polyoxyethylene compounds contain neutral head groups and a tail of hydrophobic chains, whereas glycosidic detergents usually use a sugar as a head base, such as glucose, and have an alkyl polymer tail.

Non-ionic biological detergents are less harsh than their ionic counterpart, and therefore are better used when protein structure needs to remain intact. They are weak against protein-protein bonds.

Zwitterionic Detergents

Zwitterionic is a unique subgroup of detergents that contains both positive and negative atomic group charges in the head, making the overall charge neutral.

Zwitterionic detergents have a mix of characteristics of both ionic and non-ionic detergents, and the three groups are often compared in research studies to understand best uses for each classification.

Like non-ionic, zwitterionic detergents have a neutral net charge but are a better choice for breaking protein-protein bonds. The extent to which proteins are affected by this process is less harsh than the outcome with ionic detergents.

Important Things to Consider When Choosing Your Biological Detergent

Research beforehand is always key! There are hundreds of articles on sites such as PubMed which highlight the uses and warnings for each specific detergent out there.

Some key factors to review when selecting a biological detergent are: the intended outcome, pH and ionic charges, the physicochemical properties, different experimental conditions in the environment and samples, as well as the extent to which a protein should be denatured.

And finally, always try biodetergents that have been used successfully in targeting and isolating a protein first before moving to other types if you can!

We Stand Out - Biological Detergents for All Applications

At AG Scientific, we deliver a wide range of high quality biodetergents, alongside many other reagents, enzymes, ionophores, and more, and we tailor custom reagents to meet the needs of our consumers at an individual level.

We take the customer experience seriously and cut out unnecessary bureaucracy that might prolong an order being processed and delivered. This is because we take a direct one-to-one approach in serving our customers.

We pride ourselves in being able to offer a personalized approach within areas including medical technology and diagnostic materials, while still remaining competitive with other large life science distributors in the field.

Questions? Contact our team - we are here to support you and your research endeavors.

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  1. CHAPS | Biodetergent | CAS 75621-03-3 | AG Scientific Inc.
    CHAPS Zwitterionic, High Purity
    C-1019

    Starting at $53.04

  2. NDSB-195
    NDSB-195
    D-1089

    Starting at $85.43

  3. NDSB-201
    NDSB-201
    D-1119

    Starting at $104.86

  4. Protein Solubilizer 35, Sterile Brij-35 CAS 9002-92-0 biodetergent, Sterile Brij-35 CAS 9002-92-0 purify proteins Sterile Brij-35 CAS 9002-92-0 protein studies brij-35 proteomic application
    Protein Solubilizer 35, Sterile 10% Brij-35 ampoules
    P-1504

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  5. Protein Solubilizer 40 chemical structure | CAS 9016-45-9 | AG Scientific, Inc.
    Protein Solubilizer 40, Sterile 10% of NP-40 Ampoules
    P-1505

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  6. Sulfobetaine-12 Zwitterionic Detergent CAS [14933-08-5]diagnostic, Sulfobetaine-12 carbon nanotube biosensor
    Sulfobetaine-12
    S-1022

    Starting at $113.90

  7. Dust-Free Sodium Dodecyl Sulfate chemical structure | CAS 151-21-3 | AG Scientific, Inc.
    Dust-Free SDS (Sodium Dodecyl Sulfate)
    S-2066

    Starting at $45.89

  8. NDSB-256
    NDSB-256
    D-1121

    Starting at $97.96

  9. Triton x-114 cas 9036-19-5 biodetergent purify protein, sterile Triton x-114 biological detergent
    Protein Solubilizer X-114, Sterile 10% Triton X-114 ampoules
    P-1496

    Starting at $109.29

  10. sb-10 15163-36-7 zwitterionic biodetergent biological detergent
    Sulfobetaine-10
    S-1020

    Starting at $112.19

  11. NDSB-211
    NDSB-211
    D-1091

    Starting at $119.60

  12. TWEEN 80, CAS 9005-65-6, Biodetergent purifying proteins, sterile tween 80 biological detergent purify proteins
    Protein Solubilizer 80, Sterile 10% Tween 80 ampoules
    P-1498

    Starting at $91.61

  13. Sulfobetaine-8
    Sulfobetaine-8
    S-1018

    Starting at $149.00

  14. Sulfobetaine-14
    Sulfobetaine-14
    S-1024

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  15. Sulfobetaine-16
    Sulfobetaine-16
    S-1026

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  16. Protein Solubilizer X-100 chemical structure | CAS 9002-93-1 | AG Scientific, Inc.
    Protein Solubilizer X-100, Sterile 10% Triton X-100 ampoules
    P-1500

    Starting at $109.29

  17. n-Octyl-β-D-Glucopyranoside | CAS 29836-26-8 | Biodetergent | AG Scientific, Inc.
    n-Octyl-Beta-D-Glucopyranoside (OG)
    O-1036

    Starting at $252.17

  18. Protease Inhibitor Cocktail I | AEBSF,Aprotinin, E-64, EDTA,Leupeptin
    Protease Inhibitor Cocktail I
    P-1510

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  19. Protease Inhibitor Cocktail II | Inhibitors | AG Scientific - proteomic biomarker preservation agent contains AEBSF.HCl Bestatin E-64 EDTA Pepstatin A
    Protease Inhibitor Cocktail II
    P-1511

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  20. Protease Inhibitor Cocktail III | Inhibitors | AG Scientific
    Protease Inhibitor Cocktail III
    P-1512

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  21. Protease Inhibitor Cocktail IV | Inhibitors | AG Scientific
    Protease Inhibitor Cocktail IV
    P-1513

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  22. Protease Inhibitor Cocktail V, EDTA-free | Inhibitors | AG Scientific
    Protease Inhibitor Cocktail V, EDTA-Free
    P-1514

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  23. Protease Inhibitor Cocktail VI (General Use) | AG Scientific
    Protease Inhibitor Cocktail VI
    P-1515

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  24. Phosphatase Inhibitor Cocktail I | Inhibitors | AG Scientific
    Phosphatase Inhibitor Cocktail I
    P-1517

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  25. Phosphatase Inhibitor Cocktail II | Prevents dephosphorylation | AG Sci
    Phosphatase Inhibitor Cocktail II, Solution
    P-1518-SOL

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  26. Protease Inhibitor Cocktail I, Animal Free | AG Scientific
    Protease Inhibitor Cocktail I, Animal Free
    P-1540

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  27. Protease Inhibitor Cocktail III, Animal-Free | AG Scientific - AEBSF Aprotinin, Recombinant (Animal-Free) Bestatin E-64 Leupeptin Hemisulfate and Pepstatin A
    Protease Inhibitor Cocktail III, Animal-Free
    P-1542

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  28. Protease Inhibitor Cocktail III, Animal-Free, DMSO-Free - AG Scientific
    Protease Inhibitor Cocktail III, Animal-Free, DMSO-Free
    P-1543

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  29. Protease Inhibitor Cocktail V, EDTA-Free, Animal-Free | AG Scientific
    Protease Inhibitor Cocktail V, EDTA-Free, Animal-Free
    P-1544

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  30. Protease Inhibitor Cocktail VI, Plant Cells | Plant Extracts | AG Sci
    Protease Inhibitor Cocktail VI, for Plant Cells
    P-1545

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  31. Protease Inhibitor Cocktail VII  | For HIS / TAG Proteins | AG Sci
    Protease Inhibitor Cocktail VII (for His Tag Sequences)
    P-1546

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  32. Protease Inhibitor Cocktail VII, No-DMSO | HIS / TAG, Solvent free
    Protease Inhibitor Cocktail VII, DMSO-Free (for His Tag Sequences)
    P-1547

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  33. Protease Inhibitor Cocktail VIII | Cysteine Protease | AG Scientific
    Protease Inhibitor Cocktail VIII
    P-1548

    Starting at $68.34

  34. Phosphatase Inhibitor Cocktail III | AG Scientific
    Phosphatase Inhibitor Cocktail III
    P-1549

    Starting at $98.91

  35. Phosphatase Inhibitor Cocktail IV
    Phosphatase Inhibitor Cocktail IV
    P-1550

    Starting at $90.18

  36. Serine Protease Inhibitor Cocktail I inhibits serine proteases contains AEBSF. HCl, 420 nM Aprotinin, 20 µM Elastatinal and 1µM GGACK.
    Serine Protease Inhibitor Cocktail I
    P-1516

    Starting at $97.46

  37. n-Dodecyl-Beta-D-maltopyranoside
    n-Dodecyl-Beta-D-maltopyranoside
    D-1304

    Starting at $176.38

  38. PBS-Tween Tablets (1000 mL)
    PBS-Tween Tablets (1000 mL)
    P-2387

    Starting at $132.00

  39. NP-40 (10% in H2O)
    NP-40 (10% in H2O)
    N-2366

    Starting at $119.00

  40. SDS Solution (10% in Water) | CAS 151-21-3 | Biodetergent | AG Scientific, Inc.
    SDS Solution (10% in Water)
    S-2428

    Starting at $95.00

  41. Protease Inhibitor Cocktail VI, DETA-Free | AG Scientific, Inc.
    Protease Inhibitor Cocktail VI, EDTA-Free
    P-1554

    Starting at $39.86

  42. Digitonin | CAS 11024-24-1 | Steroid Glycoside | Detergent | AG Sci
    Digitonin
    D-2425

    Starting at $60.00

  43. Sodium Dodecyl Sulfate (SDS) chemical structure | CAS 151-21-3 | AG Scientific, Inc.
    Sodium Dodecyl Sulfate (SDS), Powder
    S-2451

    Starting at $54.00

  44. Turbocharged Protease Inhibitor Cocktail
    Turbocharged Protease Inhibitor Cocktail
    T-2492

    Starting at $91.13

  45. Turbocharged Protease + Phosphatase Inhibitor Cocktail, EDTA-Free
    Turbocharged Protease + Phosphatase Inhibitor Cocktail, EDTA-Free
    T-2496

    Starting at $94.08

  46. Turbocharged Protease + Phosphatase Inhibitor Cocktail
    Turbocharged Protease + Phosphatase Inhibitor Cocktail
    T-2495

    Starting at $91.13

  47. Turbocharged Phosphatase Inhibitor Cocktail
    Turbocharged Phosphatase Inhibitor Cocktail
    T-2494

    Starting at $91.13

  48. Turbocharged Protease Inhibitor Cocktail, EDTA-Free
    Turbocharged Protease Inhibitor Cocktail, EDTA-Free
    T-2493

    Starting at $91.13

  49. Triton X Non-Ionic Detergent EIA
    Triton X Non-Ionic Detergent EIA
    T-1056

    Starting at $0.00

  50. ASB-14
    ASB-14
    A-2187

    Starting at $165.17

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