Non-ionic detergents contain uncharged, hydrophilic head groups that consist of eithermicelle polyoxyethylene moieties as in TWEEN and TRITON or glycosidic groups as in octyl glucoside and dodecyl maltoside. In general, non-ionic detergents are better suited for breaking lipid-lipid and lipid-protein interactions than protein-protein interactions.
Non-Ionic Protein Solubilizers (Non-Ionic Detergents)
Non-ionic detergents contain uncharged, hydrophilic head groups that consist of eitherpolyoxyethylene moieties as in Tween and Triton or glycosidic groups as in octyl glucoside and dodecyl maltoside. In general, non-ionic detergents are better suited for breaking lipid-lipid and lipid-protein interactions than protein-protein interactions. Hence, they are considered non-denaturant and are widely used in the isolation of membrane proteins in their biologically active form.|
Feature |
Benefit |
| Very low contaminating Aldehyde and Peroxide concentration < 0.1 mM | To maximize yields of biologically active proteins |
| Extremely low conductivity < 5 µmhos | Prevents problems associated with interfering salts and metal ions |
| 10% solution in sterile water for injection | High purity and ease of dispensing of diluted solutions |
| Pre-scored 5 ml Amber Ampoules | Small package size with amber ampoules to maximize shelf life |
| Sterile, U.S.P Grade | To maintain biological purity, Improved stability. |
| Aseptically manufactured and filled, Large Scale GMP capabilities | For Research use through therapeutic Production capabilities |
TABLE -1: Properties of Non-Ionic Detergents
| Product |
Cat.No. |
M.W.a |
CMCb (mM) |
Agg. No. |
Avg. Micellar Wt. |
HLB No. |
Cloud Point |
| Protein Solubilizer 80(10% Soln of Tween 80) |
P-1498 |
1310 |
0.012 |
58 |
76,000 |
15 |
- |
| Protein Solubilizer 20(10% Soln of Tween 20) |
P-1502 |
1228 |
0.059 |
- |
- |
16.7 |
- |
| Protein Solubilizer X-100(10% Soln of Triton X-100) |
P-1500 |
625 |
0.2-0.9 |
140 |
80,000 |
13 |
64ºC |
| Protein Solubilizer X-114(10% Soln of Triton X-114) |
P-1496 |
427 |
0.35 |
- |
- |
12.4 |
22ºC |
| Protein Solubilizer 35(10% Soln of Brij-35) |
P-1504 |
1198 |
0.09 |
40 |
48,000 |
16.9 |
- |
| Protein Solubilizer 40(10% Soln of NP-40) |
P-1505 |
603 |
0.05-0.3 |
- |
- |
13.1 |
80ºC |
| Protein Solubilizer Set-1(5 Sample Set: 2X5 ml each) |
P-1503 |
- |
- |
- |
- |
- |
- |
References
- Banerjee, P., et al. (1993). Differential solubilization of membrane lipids by detergents. Arch. Biochem. Biophys. 305, 68.
- Neugebauer, J. M. (1990). Detergents: An overview. Methods. Enzymol. 182, 239.
- Furth, A. H., et al. (1984). Separating detergents from proteins. Methods. Enzymol. 104, 318.
- Hjelmeland, L. M., and Chrambach, A. (1984). Solubilization of functional membrane proteins. Methods. Enzymol. 104, 305.
- Ashani, Y. and Catravas, G. (1980). Highly reactive impurities in Triton X-100 and Brij 35: partial characterization and removal. Anal. Biochem. 109, 55-62.
- Chang, H. W. and Bock E. (1980). Pitfalls in the use of commercial nonionic detergents for the solubilization of integral membrane proteins: sulfhydryl oxidizing contaminants and their elimination. Anal. Biochem. 104, 112-117.
- Helenius, A., McCaslin, D. R., Fries, E., and Tanford, C. (1979). Properties of detergents. Methods. Enzymol. 56, 734-749.
- Lever, M. (1977). Peroxides in detergents as interfering factors in biochemical analysis. Anal. Biochem. 83, 274.
- Slinde, E., and Flatmark, T. (1976). Effect of the hydrophile-lipophile balance of non-ionic detergents on the solubilization of biological membranes and their integral b-type cytochromes. Biochim. Biophys. Acta. 455, 796.
- Helenius, A. and Simons, K. (1975). Solubilization of membranes by detergents. Biochim. Biophys. Acta. 415, 29-79
- Triton is a registered trademark of Union Carbide.