Bovine Serum Albumin (Fraction V)

SKU
B-3371
  • CAS:9048-46-8
  • MW:~66 kDa
  • Appearance:White to yellow with tan to green cast lyophilized powder
  • Purity:≥98%

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Product Name Qty
Bovine Serum Albumin
100 g
$135.00
Bovine Serum Albumin
1 kg
$1,099.99
Bovine Serum Albumin
50 g
$74.99

Description

Bovine serum albumin (BSA) is a high-purity albumin protein derived from the blood plasma of cows. Among its most common research uses is the culturing of mammalian cells, an essential function for many biochemical, pharmacological and biological studies. In addition to its use as a culturing agent, significant research has been conducted into BSA itself. Of particular importance has been inquiry into BSA present in cow's milk as a possible causative agent of insulin-dependent diabetes mellitus. Nanoparticles of albumin have been explored as drug delivery systems due to their biocompatible nature. BSA can also be used as a diluting component in vaccines.

Applications

• Blocking agent used for ELISA and blotting procedures
• Electron and immunoelectron microscopy
• Isolation and culture of neonatal rat-cardiac ventricular myocytes
• Vaccine diluent

Specifications

• pH (5% water) @ 25ºC: 6.5-7.5
• Endotoxin: 10 EU/mg
• Molecular biology grade
• Fatty acid-free
• IgG-free
• Low bioburden
• Protease-free
• Solvent-free extraction process
• Heat shock >65ºC with Na Caprylate

 

More Information
Alternate Name/Synonyms
Albumin bovine serum; BSA; Bovine albumin; Cohn Fraction V
SKU
B-3371
CAS #
9048-46-8
Molecular Weight
~66 kDa
Appearance
White to yellow with tan to green cast lyophilized powder
Purity
≥98%
Solubility
Water: clear to slightly hazy faint yellow to yellow green solution
Storage Temp
2-8°C
THERAPEUTIC AREA
Not Applicable
USE
Bovine serum albumin (also known as BSA or "Fraction V") is a serum albumin protein derived from cows. It is often used as a protein concentration standard in lab experiments. BSA has numerous biochemical applications including ELISAs (Enzyme-Linked Immunosorbent Assay), immunoblots, and immunohistochemistry. Because BSA is a small, stable, moderately non-reactive protein, it is often used as a blocker in immunohistochemistry. During immunohistochemistry, which is the process that uses antibodies to identify antigens in cells, tissue sections are often incubated with BSA blockers to bind nonspecific binding sites. This binding of BSA to nonspecific binding sites increases the chance that the antibodies will bind only to the antigens of interest. The BSA blocker improves sensitivity by decreasing background noise as the sites are covered with the moderately non-reactive protein.
Handling
Use in a chemical fume hood, with air supplied by an independent system. Avoid inhalation, contact with eyes, skin and clothing. Avoid the formation of dust and aerosols. Use in a well-ventilated area. Keep away from sources of ignition. Avoid prolonged or repeated exposure.
Handling Use in a chemical fume hood, with air supplied by an independent system. Avoid inhalation, contact with eyes, skin and clothing. Avoid the formation of dust and aerosols. Use in a well-ventilated area. Keep away from sources of ignition. Avoid prolonged or repeated exposure.
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