E-64
SKU
E-2030
- CAS:66701-25-5
- Formula:C15H27N5O5
- MW:357.4 Da
- Appearance:White powder
- Purity:≥99%
SDS
CofA
Description
E-64 is an Irreversible, potent and highly selective inhibitor of cysteine proteases. It does not affect cysteine residues in other enzymes. E64 acts by forming a thioether bond with the thiol of the active cysteine. E-64 will not inhibit serine proteases (except trypsin) but inhibits activation-induced programmed cell death and restores defective immune responses in HIV. Specific active site titrant.
Applications
• Used as an inhibitor for calpain, papain, and cathepsin B, cathepsin L
• Used to study excystation in Giardia lamblia
• Used to reduce inflammation in cystic fibrosis patients
More Information
Alternate Name/Synonyms
Thiol protease inhibitor
SKU
E-2030
CAS #
66701-25-5
Chemical Name
(2S,3S)-3-[[(2S)-1-[4-(diaminomethylideneamino)butylamino]-4-methyl-1-oxopentan-2-yl]carbamoyl]oxirane-2-carboxylic acid
Chemical Formula
C15H27N5O5
Molecular Weight
357.4 Da
Appearance
White powder
Purity
≥99%
Solubility
Up to 20mg/ml in water, Up to 25mg/ml in DMSO
Melting Point
N/A
Preparation
N/A
Storage Temp
-20°C, desiccated
THERAPEUTIC AREA
N/A
USE
E-64 is a broad spectrum cysteine proteinase and calpain activation inhibitor. E-64 has been reported to inhibit intracellular cathepsin B, cathepsin L, cathepsin H, Bax protease activity, and reduces oxidative stress, which includes a decrease in MDA levels, ICAM-1 expression and MOP acitivity. Additionally, Prevotella intermedia studies report that E-64 blocks the breakdown of methaemoglobin and the oxidation of oxyhaemoglobin by InpA. E-64 has also been used to study excystation in Giardia lamblia. E-64 is an inhibitor of Calpain, cathepsin K and cathepsin S.
Merck Index
N/A
MDL NUMBER
MFCD00080261
CHEMACX
X1036521-8
PubChecm ID
123985
INCHI
LTLYEAJONXGNFG-DCAQKATOSA-N
SMILES
CC(C)CC(C(=O)NCCCCN=C(N)N)NC(=O)C1C(O1)C(=O)O
RTECS
RR0390000
Handling
Avoid contact with skin, eyes or clothing. Use personal protective equipment as required. Wash contaminated clothing before reuse. Do not breathe dust/fume/gas/mist/vapors/spray. Do not eat, drink or smoke when using this product. Use with local exhaust ventilation. Handle in accordance with good industrial hygiene and safety practice. Corrosive hazard. Wear protective gloves/clothing and eye/face protection.
Citations
α-Actinin-4/FSGS1 is required for Arp2/3-dependent actin assembly at the adherens junction
Vivian W. Tang and William M. Brieher
J. Cell Biol., Jan 2012; 196: 115 - 130.
Citations_Hyperlink
http://jcb.rupress.org/cgi/reprint/196/1/115
Certificate of Analysis 1
Certificate of Analysis 2
Certificate of Analysis 3
| GHS PICTOGRAMS |
|
|---|---|
| Handling | Avoid contact with skin, eyes or clothing. Use personal protective equipment as required. Wash contaminated clothing before reuse. Do not breathe dust/fume/gas/mist/vapors/spray. Do not eat, drink or smoke when using this product. Use with local exhaust ventilation. Handle in accordance with good industrial hygiene and safety practice. Corrosive hazard. Wear protective gloves/clothing and eye/face protection. |
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