G-418 Sulfate Solution, Sterile-Filtered

SKU
G-1035
  • CAS:108321-42-2
  • Formula:C20H40O10N4 • 2H2SO4
  • MW:692.7 Da
  • Appearance:Clear and colorless solution
  • Purity:≥90% (TLC)

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Starting at $37.97

Product Name Qty
G-418.Sulfate, Sterile-Filtered Solution
5x10 ml
$150.77
G-418.Sulfate, Sterile-Filtered Solution
100 ml
$251.26
G-418.Sulfate, Sterile-Filtered Solution
10 ml
$37.97
G-418.Sulfate, Sterile-Filtered Solution
50 ml
$134.01

*AG Scientific's G-418 sulfate solution is a sterile filterate solution.*

G-418 sulfate, also known as geneticin, is an aminoglycoside antibiotic similar in structure to gentamicin B1. It is commonly used in plant and mammalian cell culture as a selectable marker. G418 disulfate is freely soluble in water. Bacteria and algae require 5 µg/ml or less while animal cells may require 300-500 µg/ml.  It functions by causing a chain of nonsense mutations during translation, ultimately resulting in inhibition of polypeptide synthesis and protein elongation.

Resistance to G-418 is conferred through the nptll gene in plant cells and the neo gene in mammalian cells, which encode aminoglycoside 3'-phosphotransferase. For gene selection applications, a kill curve can be made to determine the minimum effective G418 concentration to kill non-resistant cells. G418 longstanding application as a gene selection antibiotic, has a powerful new role in Genome editing utilizing the CRISPR/Cas9 system.

 Purity data is for a concentration of 50mg/ml active.

 

More Information
Alternate Name/Synonyms
Geneticin disulfate; G418 sulfate; G-418 Solution; G418 disulphate salt
SKU
G-1035
CAS #
108321-42-2
Chemical Name
(2S,3S,4S,5S)-2-[(1S,2S,3R,4S,6R)-4,6-diamino-3-[(2S,3R,4R,5S,6R)-3-amino-4,5-dihydroxy-6-(1-hydroxyethyl)oxan-2-yl]oxy-2-hydroxycyclohexyl]oxy-5-methyl-4-(methylamino)oxane-3,5-diol;sulfuric acid
Chemical Formula
C20H40O10N4 • 2H2SO4
Molecular Weight
692.7 Da
Appearance
Clear and colorless solution
Purity
≥90% (TLC)
Solubility
Colorless solution (100mg/mL in H2O)
Melting Point
N/A
Preparation
N/A
Storage Temp
Store at 4°C
THERAPEUTIC AREA
Antibacterial
USE
Geneticin (G418) Sulfate is an aminoglycoside antibiotic that has originally been derived from Micromonospora rhodoranga NRRL 5326. This compound has been shown to cause a bypass of nonsense mutations during translation. Experiments have shown that geneticin sulfate inhibits the incorporation of amino acids into protein and can destroy fibroblasts. Mechanistic studies suggest that this agent affects the 80S ribosome and inhibits the elongation cycle. Further studies report that geneticin sulfate is highly active against amoebae, protozoa, tapeworm, and pinworm exposed murine cells.
Merck Index
N/A
RTECS
CB9378500
Handling
Avoid contact with skin, eyes or clothing. Use personal protective equipment as required. Wash contaminated clothing before reuse. Do not breathe dust/fume/gas/mist/vapors/spray. Do not eat, drink or smoke when using this product. Use with local exhaust ventilation. Handle in accordance with good industrial hygiene and safety practice. Corrosive hazard. Wear protective gloves/clothing and eye/face protection.
Certificate of Analysis 1
Certificate of Analysis 2
Certificate of Analysis 3
GHS PICTOGRAMS
Handling Avoid contact with skin, eyes or clothing. Use personal protective equipment as required. Wash contaminated clothing before reuse. Do not breathe dust/fume/gas/mist/vapors/spray. Do not eat, drink or smoke when using this product. Use with local exhaust ventilation. Handle in accordance with good industrial hygiene and safety practice. Corrosive hazard. Wear protective gloves/clothing and eye/face protection.
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