G-418 Sulfate, Sterile-Filtered Solution, 5X10 ML

AG Scientific's G-418 Sulfate Solution is a sterile filterate solution.

 

G-418 sulfate, also known as geneticin, is an aminoglycoside antibiotic similar in structure to gentamicin B1. It is commonly used in plant and mammalian cell culture as a selectable marker. G418 disulfate is freely soluble in water.

Bacteria and algae require 5 µg/mL or less while animal cells may require 300-500 µg/mL.  It functions by causing a chain of nonsense mutations during translation, ultimately resulting in inhibition of polypeptide synthesis and protein elongation.
 

Resistance to G-418 is conferred through the nptll gene in plant cells and the neo gene in mammalian cells, which encode aminoglycoside 3'-phosphotransferase.

For gene selection applications, a kill curve can be made to determine the minimum effective G418 concentration to kill non-resistant cells.