Carbenicillin Disodium Salt, 100 G

Carbenicillin Disodium Salt is a penicillin-derived β-lactam antibiotic that interferes with cell wall synthesis, primarily in gram-negative bacteria such as Pseudomonas aeruginosa, while showing low toxicity to plant tissues.

Carbenicillin acylates the penicillin-sensitive transpeptidase C-terminal area by opening the lactam ring. This inactivation intercepts the cross-linkage of peptidoglycan strands, by impeding the third and last phase of bacterial cell wall synthesis. This prompts inadequate bacterial cell wall synthesis and causes cell lysis.

Applications:

• Used in selection protocols over ampicillin due to its increased stability
• Used in genetic transformation applications to select for AmpR transformed cells
• Used to regulate bacterial growth in plants, yielding low regeneration frequencies
• Used as a gene selection antibiotic in plant biology utilizing the CRISPR/CAS9 system