Heparin 4B is an affinity matrix widely used in affinity purification of various heparin-binding proteins or ligands, such as antithrombin III, lipoprotein, DNA binding proteins (transcription factors, virus coat proteins etc.) and steroid receptors. It can also be used as a high capacity cation exchange medium.
Specific proteins can be separated by using different concentrations of salt or a salt gradient. Heparin- exhibits excellent binding capacity, a high flow rate, and no significant loss of the heparin ligand. Heparin 4B contains heparin (from porcine intestinal mucosa) immobilized by CNBr activation coupling method.
Supplied as suspension in 0.5 M NaCl
- Ligand: Porcine heparin
- Matrix: 4B (agarose beads, 4%)
- Particle size range: 52 – 165 µm
- Molecular weight range: 6 x 104 – 2 x 107
- Ligand coupling method: CNBr activation
- Ligand density: 5mg heparin / ml gel
- Binding capacity: ~ 2 mg bovine antithrombin / ml gel
- pH stability: 2 – 11 (ligand dependent)
Research or further manufacturing use only, not for food or drug use.