50 kDa (SDS-PAGE), 160 kDa (gel filtration)
Diaphorase are dehydrogenase enzymes which catalyze the dehydrogenation of the reduced forms of the co-enzyme NADH and NADPH. NADH and NADPH originally come from purified extracts of Clostridium kluyveri cells, where diaphorase activity is shown. Other diaphorase activities come from various bacteria, plants and mammalian organs. This enzyme was isolated from Bacillus megaterium.
Applications:
• Used in the reoxidation of NADP to NADPH
• Used to construct a biosensor for acetaldehyde by immobilization
• Used in a study to assess the protein-protein interactions in assembly of lipoic acid on the 2-oxoacid dehydrogenases of aerobic metabolism
Research or further manufacturing use only, not for food or drug use.
