-20°C
FastPANGEA™ Long PCR DNA Polymerase is an accurate and robust enzyme that combines Horse-Power™ Taq DNA Polymerase (H-2729) and a DNA proofreading Polymerase with 3’ to 5’ exonuclease activity that is optimized for PCR amplification of very long DNA templates (long range PCR).
As it is already well-known, Taq DNA Polymerase is inefficient at amplifying fragments larger than 3–5 kb due to its inability to repair nucleotide mismatches following misincorporation. The addition of a small quantity of proofreading enzyme allows mismatches to be repaired and extension to continue, resulting in the amplification of long amplicons with high yield. The presence of the proofreading polymerase significantly increases fidelity (6.5x) as compared to Taq DNA Polymerase alone. This mixture of enzymes allows for long and accurate PCR amplification of targets from a variety of templates, such as 5-15 kb of genomic DNA.
It generates long templates with an accuracy and speed previously unattainable with other thermostable DNA polymerases. As well, it possesses 3´→ 5´ exonuclease activity and it generates PCR products with blunt ends and generate 3´-adenine overhang in amplified DNA and thus such Taq amplified DNA could be cloned into T-vectors.
Advantages & Features:
• High Fidelity: 6.5 times higher fidelity than Taq DNA Polymerase alone
• Robust: PCR amplification of fragments up to 15 kb such as 5-15 kb of genomic DNA
• High Yield: increased amplicon specificity and yield
• Accurate
• Versatile: proven performance for long or difficult templates
Applications:
• PCR-Cloning
• Primers extension
• Long or difficult amplification
• High-Throughput PCR
Specifications:
• Concentration: 5 U/μL
• One unit is defined as the amount of enzyme that incorporates 10 nmoles of dNTP’s into acid-insoluble form in 30 minutes at 75 °C under assay conditions: 25 mM TAPS-HCl, pH 9.0 (at 25 °C), 100 mM KCl, 1.5 mM MgCl2, 1 mM Beta-mercaptoethanol, 200 μM each dNTP and 10 μg activated calf thymus DNA in 50 μl.
• Free of bacterial DNA (by qPCR)
*Developed and manufactured by Canvax Biotechnology, S.L under contractual agreement with AG Scientific.
0.1 lbs
Research or further manufacturing use only, not for food or drug use.
