-20°C
HotBegan™ Hot Start Taq DNA Polymerase is a specific, highly efficient and sensitive HotStart DNA Polymerase designed to minimize unspecific amplification, improving PCR specificity. It is a Horse-Power™ Taq DNA Polymerase bound to a proprietary antibody that blocks polymerase activity until a denaturation step occurs. The heat-labile antibodies are rapidly inactivated by raising the temperature (4 minutes at 95-97 °C). This prevents or minimizes primer-dimer and non-specific products.
Like Horse-Power™ Taq DNA Polymerase (H-2729), the enzyme has 5’→3’ polymerase activity and a weak 5’→3’ exonuclease activity but no 3’→5’ exonuclease activity (proofreading). Before enzyme activation none of the enzyme activities are detectable.
Advantages & Features:
• High specificity: minimizes unspecific amplification
• Efficient: prevents or minimizes primer-dimer and nonspecific products
• Great sensitivity: amplifies from femptograms of DNA targets
• Inactive at room temperature
• Optimized: adds extra nucleotides (preferentially adenine) without template at 3´ends leaving 3´overhangs PCR fragments
• Powerful: amplification of targets up to 5 kb
• Convenient: available in kits and MasterMix solution
Applications:
• qPCR
• PCR and RT-PCR
• Genotyping with Taqman Probes
• PCR fragments amplification for TA or GC cloning
• Amplification from a limited DNA template or low copy number genes
Specifications:
0.1 lbs
Research or further manufacturing use only, not for food or drug use.
