30827-99-7
Water
-20°C
Protease Inhibitor Cocktail, EDTA-Free Tablets are designed to protect isolated proteins by providing an extraordinary inhibition of serine proteases, cysteine proteases, and aminopeptidases during extractions from animal and plant tissues or cells, yeast, and bacteria.
The provided tablets do not contain EDTA, leaving the stability and the function of metal-depended proteins unaffected. Contains both irreversible and reversible protease inhibitors. Aspartic and metallo proteases are not inhibited with the exception of aminopeptidases.
The affinity purification of Poly-His tagged fusion proteins via IMAC (immobilized metal ion affinity chromatography) is also facilitated (no dialysis necessary).
Tablets contain AEBSF, Aprotinin, E-64, and Leupeptin hemisulfate.
Preparation: Dissolve tablets directly in the extraction medium. Depending on the proteolytic activity, one tablet should be sufficient for the inhibition of the existing proteolytic activity in 50 mL. If very high proteolytic activity is present, one tablet should be used for 25 mL extraction buffer.
Research or further manufacturing use only, not for food or drug use.
