Tris Base

SKU
T-1178
  • CAS:77-86-1
  • Formula:C4H11NO3
  • MW:121.1 Da
  • Appearance:White crystalline powder
  • Purity:≥ 99.8 % (dried basis GC)

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Starting at $74.99

Product Name Qty
TRIS Base
1 kg
$74.99
TRIS Base
5 kg
$309.99
TRIS Base
25 Kg
$1,429.99

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Description

Tris (Hydroxymethyl) Aminomethane (Tris) is a titrating agent in vivo as well as in vitro. Tris buffer has been widely used in studies concerning the gram negative bacteria cell envelope. Such studies have revealed that the effect of Tris on the outer membrane appears to be highly specific. It has been suggested that Tris alters the outer membrane permeability by disrupting ionic interactions.

Recent work related to antigen retrieval agents has shown that Tris significantly reverses formaldehyde fixation of Angiotensin 1 demonstrated by reduction of the mono-methylene (m/z 1308 Da) and di-methylene adduct (m/z 1320 Da) and regeneration of the native species (m/z 1296 Da) compared to water alone. 

Studies have provided evidence that implies Tris does not act as a simple buffer inside the cell but most likely interacts with enzyme systems. Tris buffer is a very useful tool for the physiologists and pharmacologists who wish to study the effects of acid-base balance alterations. it has also been helpful in clinical medicine.

Specifications

• EC Number: 201-064-4
• Moisture: <0.2%
• Color: <20 APHA (colorless)
• Heavy Metals (as Pb): <0.0001%
• Insoluble Matter: <0.005%
• Heavy Metals: 5 ppm
• pKa (20°C): 8.1 ± 0.1
• pH (1M in water): 10.5 - 11.5

 

More Information
Alternate Name/Synonyms
Tris(hydroxymethyl)aminomethane; tromethamine; Tham; Trizma; Trometamol
SKU
T-1178
CAS #
77-86-1
Chemical Name
2-amino-2-(hydroxymethyl)propane-1,3-diol
Chemical Formula
C4H11NO3
Molecular Weight
121.1 Da
Appearance
White crystalline powder
Purity
≥ 99.8 % (dried basis GC)
Solubility
Water
Melting Point
170-172 °C
Preparation
N/A
Storage Temp
Room temperature, desiccated
THERAPEUTIC AREA
Not Applicable
USE
Tris base has been used in the dissection medium and culture medium of Hippocampal organotypic tissue cultures. It is a component of lysis buffer which is used before immunoprecipitation and immunoblotting, and a component of NuPAGE loading buffer for SDS-PAGE and as a component of transfer buffer for Western blotting. Tris buffer solutions are widely used in cell and molecular biology for processes such as protein and nucleic acid extraction and purification. Trizma based buffers are also in column chromatography and in gel electrophoresis.
Merck Index
N/A
MDL NUMBER
MFCD00004679
CHEMACX
X1001557-9
PubChecm ID
6503
INCHI
InChI=1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2
SMILES
C(C(CO)(CO)N)O
RTECS
TY2900000
Handling
Use in a chemical fume hood, with air supplied by an independent system. Avoid inhalation, contact with eyes, skin and clothing. Avoid the formation of dust and aerosols. Use in a well-ventilated area. Keep away from sources of ignition. Avoid prolonged or repeated exposure.
Certificate of Analysis 1
Certificate of Analysis 2
Certificate of Analysis 3
GHS PICTOGRAMS
Handling Use in a chemical fume hood, with air supplied by an independent system. Avoid inhalation, contact with eyes, skin and clothing. Avoid the formation of dust and aerosols. Use in a well-ventilated area. Keep away from sources of ignition. Avoid prolonged or repeated exposure.
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