Leupeptin Hemisulfate

SKU

L-1165

CAS:103476-89-7
Formula:C₄₀H₇₈N₁₂O₁₂S
MW:951.2 Da
Appearance:White powder
Purity:>90% (HPLC)

SDS

CofA

Starting at $30.60

Product Name	Qty
Leupeptin Hemisulfate 5 mg $30.60
Leupeptin Hemisulfate 25 mg $86.70
Leupeptin Hemisulfate 500 mg $561.00
Leupeptin Hemisulfate 100 mg $255.00
Leupeptin Hemisulfate 10 mg $56.10

Details

Description

Leupeptin is a reversabile protease inhibitor known to target various cathepsins, calpain and trypsin. In biochemical research, leupeptin is used frequently as a reducing agent in experiments designed to study proteins, enzymes, amino acids and neuropeptides.

Applications

While it is used widely as a reagent, several studies have been conducted into leupeptin's direct biomedical effects. In one such study, leupeptin was shown to protect hair cells in the inner ear from ototoxicity associated with aminoglycoside antibiotic treatment. In rat studies, leupeptin has demonstrated a considerable ability to suppress inflammation caused by gingivitis.

Leupeptin has also played a role in studies of certain cancers for several decades. In the early 1970s, the compound was found to have an inhibitory effect on tumorigenesis of skin cancer in mice. More recently, it has been used in studies relating to the role of the protease matriptase in the development of breast cancers.

Leupeptin Solution Protocol

Leupeptin Hemisulfate in methanol stock solution can be prepared at concentration of 20 mg/mL. Store at stock solution at -20°C.

Specifications

More Information

Alternate Name/Synonyms

Leupeptin hemisulfate salt; Leupeptin hemisulfate anhydrous

SKU

L-1165

CAS #

103476-89-7

Chemical Name

(2S)-2-acetamido-N-[(2S)-1-[[(2S)-5-(diaminomethylideneamino)-1-oxopentan-2-yl]amino]-4-methyl-1-oxopentan-2-yl]-4-methylpentanamide;sulfuric acid

Chemical Formula

C₄₀H₇₈N₁₂O₁₂S

Molecular Weight

951.2 Da

Appearance

White powder

Purity

>90% (HPLC)

Solubility

Soluble in water (50 mg/mL), clear and colorless to faint yellow solution

Storage Temp

-20°C

THERAPEUTIC AREA

Not Applicable

USE

It is often used during in vitro experiments when a specific enzymatic reaction is being studied. When cells are lysed for these studies, proteases, many of which are contained within lysosomes, are released. These proteases, if freely present in the lysate, would destroy any products from the reaction being studied, and make the experiment uninterpretable. For example, leupeptin could be used in a calpain extraction to keep calpain from being hydrolyzed by specific proteases.

INCHI

InChI=1S/2C20H38N6O4.H2O4S/c2*1-12(2)9-16(24-14(5)28)19(30)26-17(10-13(3)4)18(29)25-15(11-27)7-6-8-23-20(21)22;1-5(2,3)4/h2*11-13,15-17H,6-10H2,1-5H3,(H,24,28)(H,25,29)(H,26,30)(H4,21,22,23);(H2,1,2,3,4)/t2*15-,16-,17-;/m00./s1

SMILES

OS(O)(=O)=O.[H]C(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC(C)C)NC(C)=O.[H]C(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC(C)C)NC(C)=O

Handling

Avoid contact. Do not get in eyes, on skin, or clothing. Wash thoroughly after handling. Wash contaminated clothing before reuse. Hygroscopic! Protect from light and moisture!

Citations

α-Actinin-4/FSGS1 is required for Arp2/3-dependent actin assembly at the adherens junction Vivian W. Tang and William M. Brieher J. Cell Biol., Jan 2012; 196: 115 - 130.

Citations_Hyperlink

http://jcb.rupress.org/cgi/reprint/196/1/115

Additional Videos

msds 1

L-1165, Leupeptin, SDS, diamond format.pdf

Certificate of Analysis 1

L-1165, L1089.pdf

Certificate of Analysis 2

L-1165, J1181C.pdf

Certificate of Analysis 3

L-1165, J1181B.pdf

Reviews

Safety

GHS PICTOGRAMS
Handling	Avoid contact. Do not get in eyes, on skin, or clothing. Wash thoroughly after handling. Wash contaminated clothing before reuse. Hygroscopic! Protect from light and moisture!

Citations

Citations	Distinctive Activation Mechanism for Angiotensin Receptor Revealed by a Synthetic Nanobody
	Angiotensin Analogs with Divergent Bias Stabilize Distinct Receptor Conformations
	CPG2 Recruits Endophilin B2 to the Cytoskeleton for Activity-Dependent Endocytosis of Synaptic Glutamate Receptors
	Heat shock proteins regulate activation-induced proteasomal degradation of the mature phosphorylated form of protein kinase C

Blog

We found other products you might like!