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Leupeptin, Hemisulfate

SKU
L-1165
  • CAS:103476-89-7
  • Formula:C40H78N12O12S
  • MW:951.2 Da
  • Appearance:White powder
  • Purity:>90% (HPLC)

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SDS
CofA

Starting at $30.00

Product Name Qty
Leupeptin, Hemisulfate
5 mg
$30.00
Leupeptin, Hemisulfate
25 mg
$85.00
Leupeptin, Hemisulfate
500 mg
$550.00
Leupeptin, Hemisulfate
100 mg
$250.00
Leupeptin, Hemisulfate
10 mg
$55.00
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Description

Leupeptin is a reversabile protease inhibitor known to target various cathepsins, calpain and trypsin. In biochemical research, leupeptin is used frequently as a reducing agent in experiments designed to study proteins, enzymes, amino acids and neuropeptides.

Applications

While it is used widely as a reagent, several studies have been conducted into leupeptin's direct biomedical effects. In one such study, leupeptin was shown to protect hair cells in the inner ear from ototoxicity associated with aminoglycoside antibiotic treatment. In rat studies, leupeptin has demonstrated a considerable ability to suppress inflammation caused by gingivitis.

Leupeptin has also played a role in studies of certain cancers for several decades. In the early 1970s, the compound was found to have an inhibitory effect on tumorigenesis of skin cancer in mice. More recently, it has been used in studies relating to the role of the protease matriptase in the development of breast cancers.

Leupeptin Solution Protocol

Leupeptin Hemisulfate in methanol stock solution can be prepared at concentration of 20 mg/mL. Store at stock solution at -20°C.

 

More Information
Alternate Name/Synonyms
Leupeptin hemisulfate salt; Leupeptin hemisulfate anhydrous
SKU
L-1165
CAS #
103476-89-7
Chemical Name
(2S)-2-acetamido-N-[(2S)-1-[[(2S)-5-(diaminomethylideneamino)-1-oxopentan-2-yl]amino]-4-methyl-1-oxopentan-2-yl]-4-methylpentanamide;sulfuric acid
Chemical Formula
C40H78N12O12S
Molecular Weight
951.2 Da
Appearance
White powder
Purity
>90% (HPLC)
Solubility
Soluble in water (50 mg/mL), clear and colorless to faint yellow solution
Melting Point
N/A
Preparation
N/A
Storage Temp
-20°C
THERAPEUTIC AREA
Not Applicable
USE
It is often used during in vitro experiments when a specific enzymatic reaction is being studied. When cells are lysed for these studies, proteases, many of which are contained within lysosomes, are released. These proteases, if freely present in the lysate, would destroy any products from the reaction being studied, and make the experiment uninterpretable. For example, leupeptin could be used in a calpain extraction to keep calpain from being hydrolyzed by specific proteases.
Merck Index
N/A
INCHI
InChI=1S/2C20H38N6O4.H2O4S/c2*1-12(2)9-16(24-14(5)28)19(30)26-17(10-13(3)4)18(29)25-15(11-27)7-6-8-23-20(21)22;1-5(2,3)4/h2*11-13,15-17H,6-10H2,1-5H3,(H,24,28)(H,25,29)(H,26,30)(H4,21,22,23);(H2,1,2,3,4)/t2*15-,16-,17-;/m00./s1
SMILES
OS(O)(=O)=O.[H]C(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC(C)C)NC(C)=O.[H]C(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC(C)C)NC(C)=O
RTECS
N/A
Handling
Avoid contact. Do not get in eyes, on skin, or clothing. Wash thoroughly after handling. Wash contaminated clothing before reuse. Hygroscopic! Protect from light and moisture!
Citations
α-Actinin-4/FSGS1 is required for Arp2/3-dependent actin assembly at the adherens junction Vivian W. Tang and William M. Brieher J. Cell Biol., Jan 2012; 196: 115 - 130.
Citations_Hyperlink
http://jcb.rupress.org/cgi/reprint/196/1/115
Additional Videos
msds 1
L-1165, Leupeptin, SDS, diamond format.pdf
Certificate of Analysis 1
L-1165, J1181C.pdf
Certificate of Analysis 2
L-1165, J1181B.pdf
Certificate of Analysis 3
L-1165, J1181A.pdf
GHS PICTOGRAMS
Handling Avoid contact. Do not get in eyes, on skin, or clothing. Wash thoroughly after handling. Wash contaminated clothing before reuse. Hygroscopic! Protect from light and moisture!
Citations Distinctive Activation Mechanism for Angiotensin Receptor Revealed by a Synthetic Nanobody
Angiotensin Analogs with Divergent Bias Stabilize Distinct Receptor Conformations
CPG2 Recruits Endophilin B2 to the Cytoskeleton for Activity-Dependent Endocytosis of Synaptic Glutamate Receptors
Heat shock proteins regulate activation-induced proteasomal degradation of the mature phosphorylated form of protein kinase C
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