Proteinase K (Recombinant), Molecular/PCR Grade

SKU
P-1264
  • MW:29.3 kDa
  • Appearance:White to off-white lyophilized solid with no additives
  • Purity:≥99%

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Product Name Qty
Proteinase K (recombinant), Molecular Grade, PCR grade
1 g
$537.62
Proteinase K (recombinant), Molecular Grade, PCR grade
10 g
$4,539.57
Proteinase K (recombinant), Molecular Grade, PCR grade
100 mg
$105.30
Proteinase K (recombinant), Molecular Grade, PCR grade
500 mg
$315.92

A highly reactive serine protease that displays the ability to digest native proteins, thereby inactivating enzymes such as DNase and RNase without recourse to a denaturation process. It is the most powerful proteinase among all proteinases characterized so far. It cleaves at the peptide bond adjacent to the carboxylic acid group of aliphatic, aromatic or hydrophobic amino acids.

The application of the molecular grade Proteinase K is similar to the Native Proteinase K and is used in the isolation or preparation of high molecular weight nucleic acids. However, this Proteinase K is highly pure and has a higher specific activity and is more stable at room temperature as compared to native Proteinase K.

It is stable and active over a wide pH range of 4-12.

Recombinant Proteinase K  is also active with SDS, urea and EDTA and the most active temperature is 65°C.

It is inactivated by diisopropyl fluorophosphates (DFP) and phenyl methane sulfonyl fluoride (PMSF).

Application

• This Recombinant Proteinase K is extremely effective on native proteins and can, therefore, be used to rapidly inactivate endogenous nucleases such as RNases and DNases. This property makes Proteinase K particularly suitable for the isolation of native RNA and DNA from tissues or cell lines.

• The enzyme promotes cell lysis by activating a bacterial autolytic factor.

• Proteinase K is also used for the analysis of membrane structures by modifying proteins and glycoproteins on cell surfaces.

• The enzyme is particularly well suited for isolating nucleic acids for amplification reactions.

• Proteinase K can be used to remove cellular debris during the preparation of colony lifts, and to treat tissue sections to ensure efficient probe infiltration during in situ hybridization.

More Information
Alternate Name/Synonyms
Protease K; Endopeptidase K; Tritirachium alkaline proteinase
SKU
P-1264
Molecular Weight
29.3 kDa
Appearance
White to off-white lyophilized solid with no additives
Purity
≥99%
Solubility
50mM Tris-HCl(pH 7.5), 3mM CaCl2, 50% Glycerol
Storage Temp
4°C, desiccated
THERAPEUTIC AREA
Not Available
USE
This PCR Grade Proteinase K is particularly suitable for isolating nucleic acids for amplification reactions, as well as for the analysis of membrane structures by modifying proteins and glycoproteins on cell surfaces. It is extremely effective on native proteins and can therefore be used to rapidly inactivate endogenous nucleases such as RNases and DNases during nucleic acid isolation. This property makes Proteinase K particularly suitable for the isolation of native RNA and DNA from tissues or cell lines. It promotes cell lysis by activating a bacterial autolytic factor. Because the enzyme is tested for the absence of RNases and DNases, and is virtually free of DNA, it is especially suitable for isolating PCR and RT-PCR templates. Proteinase K can also be used to remove cellular debris during the preparation of colony lifts, and to treat tissue sections to ensure efficient probe infiltration during in situ hybridization.
Handling
Wear personal protective equipment. Ensure adequate ventilation. Do not get in eyes, on skin, or on clothing. Avoid ingestion and inhalation. Avoid dust formation. Wash hands before breaks and immediately after handling the product.
Certificate of Analysis 1
Certificate of Analysis 2
GHS PICTOGRAMS
Handling Wear personal protective equipment. Ensure adequate ventilation. Do not get in eyes, on skin, or on clothing. Avoid ingestion and inhalation. Avoid dust formation. Wash hands before breaks and immediately after handling the product.
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